Greetings, IŽd been interested in mailing with anyone who works on platelet activation and flow citometryc assays on platelets rich plasma. I have troubles with my protocols for platelet activation. So far, I am not able to stop it. We activated with thrombine for 1 minute, and then we look for activation markers on the surface of the platelets , but we always find a fibrin clot, although EDTA is added to stop the activation step. Is anybody experienced with platelet activation? Does anybody know any other way to activate platelets without clotting? Thanks in advance. Dr. Martha Moreno. Dept. of Immunology. E.N.C.B., I.P.N. Fax (525) 396-3503
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