Concerning the refrigeration of peripheral blood samples for routine testing of T/B subsets, traditionally this was thought to induce changes in certain antigen levels. Published guidelines (i.e. "CDC MMWR) for Immunophenotyping of patient samples suggest using only samples kept at room temperature. Question (1) Is this true and are there any recent literature references? (2) Is the process reversible after the sample returns to room temperature? It wouldn't be difficult to test this but published references would be most helpful. (3) What is the current thinking about refrigeration (4-10 C) of other samples such as bone marrow, tissues, etc? Thanks for whatever references can be provided. Bruce Greig Immunopathology Laboratory Vanderbilt University Medical Center Nashville, TN. 37232 (615)322-2682 Email:Brugreig@aol.com
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