Mail*LinkŪ SMTP Clean Machines, Ad infunum.
In response to all the comments on sterilizing a Flow Cytometer.
Let me throw my two cents in.
I don't sterilize the instrument!!!!!!!!!!!!
(That is with any harsh chemicals ecetera.)
I have a BDIS FacStar Plus. It comes with a filter housing on the sheath
tank.
The manufacturer recommends first using a pre filter and then a 0.45
micron filter.
I have installed a 0.22 micron filter piggy backed on the 0.45 micron
filter.
The sheath first passes through the pre filter then through the 0.45
micron and lastly through the 0.22 micron filter.
Thus the sheath filter is now filter sterilized to 0.22 microns.
To test for sterility I turn on the Sheath only and after about five
minutes I hold a Blood agar plate to collect the
sheath stream (@ 5 mls) and place the blood plate in aerobic and/or
anaerobic conditions and haven't seen any growth yet.
When I sort I do sterilize the sample input line with bleach followed by
sterile 0.22 micron filtered sterile dH2O.
Pb
------------------ RFC822 Header Follows ------------------
Received: by amgen.com with ADMIN;25 Feb 1997 12:48:09 -0800
Received: from castle-smtp.amgen.com (castle-smtp [138.133.19.51])
by www-int.amgen.com (8.8.5/8.8.5) with SMTP id MAA29166;
Tue, 25 Feb 1997 12:34:13 -0800 (PST)
Received: by castle-smtp.amgen.com; id MAA23516; Tue, 25 Feb 1997 12:34:12 -0800
Received: from flowcyt.cyto.purdue.edu(128.210.60.31) by castle-smtp.amgen.com via smap (V3.1)
id xma023500; Tue, 25 Feb 97 12:33:46 -0800
Received: by flowcyt.cyto.purdue.edu (940816.SGI.8.6.9/930416.SGI.AUTO)
for cyto-sendout id OAA27818; Tue, 25 Feb 1997 14:55:21 -0500
Received: from [199.68.16.3] by flowcyt.cyto.purdue.edu via SMTP (940816.SGI.8.6.9/930416.SGI.AUTO)
for <cytometry@flowcyt.cyto.purdue.edu> id LAA26211; Tue, 25 Feb 1997 11:45:25 -0500
Received: from medimmune4 ([172.16.0.4]) by [199.68.16.3]
via smtpd (for flowcyt.cyto.purdue.edu [128.210.60.31]) with SMTP; 25 Feb 1997 16:37:33 UT
Received: by medimmune4.medimmune.com with SMTP (Microsoft Exchange Server Internet Mail Connector Version 4.0.995.28)
id <01BC230F.DF8072B0@medimmune4.medimmune.com>; Tue, 25 Feb 1997 11:34:34 -0500
Message-ID: <c=US%a=_%p=MedImmune._Inc.%l=MEDIMMUNE4-970225163432Z-1160@medimmune4.medimmune.com>
From: "Barren, Phil" <BarrenP@MedImmune.com>
To: cyto-inbox
Subject: Clean Machines, Ad infunum.
Date: Tue, 25 Feb 1997 11:34:32 -0500
X-Mailer: Microsoft Exchange Server Internet Mail Connector Version 4.0.995.28
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Transfer-Encoding: 7bit
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:27 EST