I have heard so much on clean machines I just have to add a touch more. If you start with a clean machine and provide consistent regular cleaning then you will probably not have a problem. For those who wish to clean only when things get dirty of contaminated then they are going to have alot of fun. Once sheath lines are contaminated by protozoa or bacteria (or even better the disconnects for tubing) the only recourse is to replace all tubing and connectors. All the decontaminates you can use will not get into every little nook and cranny. The cost for replacing all of this is less than the antibodies you will use to stain your samples. Start clean and keep it clean.(regularly) Jim Houston St. Jude Children's Research Hospital Cell and Gene Therapy
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