I have been working with BDs FastImmune anti-IFNg and anti-IL-4 antibodies for about 2 months (but only with normal controls) and have been very pleased with the results. I have been following BD's protocol and have had success with each experiment. I have used both the IFNg/IL-4 combo with the isotype combo and individual reagents with success. Although BD sells a special formulation of IgG1 PE, I have been titering their standard formulation and think it will work nearly as well as a control for IL-4 PE. I have not tried using saponin for permeablization but will be comparing BD's FACSPerm to Caltag's Fix N' Perm. If you would like more details, please email me directly. -- Michelle =========================================================================== Michelle N. Fiordalisi, Ph.D. Clinical Immunology Fellow University of North Carolina Hospitals email: mnfiord@med.unc.edu Phone: 919-966-4058 FAX: 919-966-0486 =========================================================================== On Thu, 20 Feb 1997, Vishal Gulati wrote: > > > Hi! Has anyone on the list used BD Fastimmune Intracellular cytokine > staining reagents. I have been using 'conventional' indirectly lablled > cytokine antibodies and am planning to switch over. > > Also, has someone compared saponin with BD/any other commercial > permeablising reagent . > > Id be grateful if those of you out here in the know of things share their > experiences. > > Thanks in advance > > > Bye > > > Vishal >
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