Hi, Does anyone have experience staining GFP (S65T, humanized codon, not a fusion protein)-expressing cells for BrdU incorporation? I'm afraid standard alcohol fixation and HCl treatment is too harsh for GFP. I read about nuclease treatment and UV breaking. Which anti-BrdU antibody would you recommend? In fact, is there a better way to detect proliferating cells? Is there, say, a thymidine analog that is incorporated into newly synthesized DNA and fluoresces on its own? Certain easily-detectable antigen that is "really" specific to some cell cycle stage? Mayumi Naramura, M.D. NIH/NIAID Lab of Immunology Twinbrook II, Rm. 125 12441 Parklawn Drive Rockville, MD 20852 Phone: 301-402-4595 FAX: 301-594-2522 e-mail:mnaramura@atlas.niaid.nih.gov
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