We want to test for Cyclin D1 on peripheral blood and FNA samples from patients with possible B lymphoproliferative disorders. I have bought some Immunotech Cyclin D1 (Cat No 1740) and tried the method we use for Ki-67 and cytoplasmic CD3 and CD22: Acetone fix of cytospins followed by standard 2 stage immunofluorescence. We are testing the antibody at 1/10 and 1/100 to start with. Several problems: We haven't seen any convincing positivity although we have had several possible Mantle cell lymphomas. Also what sort of staining is seen - is it similar to TdT or Ki-67? While we wait for a positive to turn up perhaps someone who has had experience with this antibody in the context of B LPD could advise if the method is inappropriate.Should we try flow? Many thanks Jan Jan Nelson Molecular Haematology Department of Molecular Medicine The University of Auckland Phone: (+64)(9) 373-7599 ext 6381 Fax: (+64)(9) 373-7292
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