Hi We too are experiencing connection problems with our FACSCalibur. However, our problems only started AFTER we got the second laser installed. The timing between when we turn on the cytometer to when we switch on the computer is well within the magical 1-5 min window. However, we often (almost every other time) have connection problems. Shutting down & restarting the computer doesn't seem to help - only a total shutdown of cytometer & computer works...most of the time (sometimes you have to do this many times). Occasionally everything connects properly but we cannot see events. The magical solution to this problem seems to be to tighten the Bal Seal, however sometimes this doesn't work. We've tried all the 'solutions' offered in the troubleshooting section & we were just wondering if anyone else has experienced this problem. Seeing the various messages on this topic makes us feel better as we had the impression that there was nothing wrong with the machines (that no one else experiences this problem) & that it was just us and our inexperience. So thank you fellow FLOWers! We have also noticed that when we switched to the PPC from the Quadra, we now get a message that says that there is an error in the connection & that the cytometer cannot be found when we only want to analyse (ie we do not turn the cytometer on). If you click OK, then you can just analyse, but before we upgraded we never had this problem! Thanks in advance for your help Maribel Leong Dept Med, University of Melbourne Australia
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