Salut tout le monde, Concerning the (spring) cleaning discussions, here are my 5 cents: For the sorters: I am using indeed denture cleansing tablets (brandname: Steradent, made somewhere in Germany, I buy it in a pharmacy) to clean my nozzles for a FACStar Plus every day, just throw them in over night, and they come out perfectly clean in the morning. However be careful of the brand you use , as some don't dissolve completely (Correga Tabs are no good...) and therefore the small particles left in there might clog the nozzle as well, also rinse + flush the nozzle carefully with dH2O before using it... Certainly one of the best cleaning solutions for the nozzles (thanks to Steve Merlin who told me this one !). FACScan Flowcells: 1. For FACScan flowcells I use in general the liquid cleaner for industrial dishwashers (diluted of course), as this is non offensive and also cleans up perfectly the flowcell... and it's cheap. Just make sure that you immediately afterwards dH2O as well... or your cells might not like it too much. 2. Now I have also tried FACSrinse, and have to say there is no difference in the amount of clogging or the residual cells/beads left in there with either of those solutions 3. However I also use a couple of drops of CIF (Lever Inc. - don't know what the equivalent brand name in other parts of the world is) diluted in 50 ml of water to clean up flowcells from top to bottom from time to time, as the flowcell is quartz the abrasive particles in there are not offensive either. Have kept my CV's really low that way... Bon hope that helps those who are looking for alternatives. Cheers, and happy cleaning, Matthias ______________________________________________________________ Matthias Haury _/_/_/ _/_/_/ _/_/_/ Flowcytometry - Immunology _/ _/ _/ _/ _/ Institut Pasteur Paris _/ _/_/_/ _/_/_/ Email: mhaury@pasteur.fr _/ _/ _/ Tel: 33 (01) 40 61 31 29 _/_/_/ _/ _/ Fax: 33 (01) 45 68 86 39 INSTITUT PASTEUR PARIS ______________________________________________________________ At 9:30 +0000 17/02/97, Peter J Openshaw [Medicine] wrote: >I have been told that denture cleansing tablets are the answer to the >ultimate clog/dirty cell. I have not tried them, but can imagine that >companies have spent millions on making the perfect combination to clean >false teeth. Has anyone tried? >--------------------------------------------------- >Peter J Openshaw [Medicine] (p.openshaw@ic.ac.uk) >Imperial College School of Medicine at St.Mary's >Norfolk Place, LONDON W2 1PG >Fax: +44 171 724 7349 Tel: +44 171 594 3854/3 >---------------------------------------------------
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