Dear Flowlanders, Can anybody help me in the matter of using TRAP-6 to activate platelets? How much TRAP do you use to maximally activate platelets? I mean how many micro mol per how many platelets? The papers I have read mention only the ammount of TRAP (usually 100uM). I had some problems with activating plts with TRAP - seems like I used too many plts, or too little TRAP, but maybe the problem is to be sought elswhere... The second question to the illuminati is how to determine the proper compensation for the WB 2 color platelet activation analysis? Many thanx, Janek ______________________________________________ Jan L. Brozek e-mail: mmbrozek@cyf-kr.edu.pl http://www.cyf-kr.edu.pl/~mmbrozek
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