Seems like we're the only ones that use detergent. We fill the tubing with a 60cc syringe with 1:100 7X detergent in millipore H2O and let it sit for an hour or two. This is followed by a millipore H2O rinse, then 60cc of 70% EtOH to help flush the detergent, followed by half a liter of PBS. The 7X and EtOH is filtered with a 0.45 micron syringe filter. Detergent is never used in the sheath tank or filter housing. We do this about once per month and it works beautifully. The only time I use bleach is if there's a known contaminant and we haven't had one of those in many years. Bleach does some pretty horrible things to the tubing not to mention your nozzle tip. good luck, td -- ============================================================================== Thomas Delohery | Internet: t-delohery@ski.mskcc.org Manager, Flow Cytometry Core Facility | Phone: (212) 639-8729 Memorial Sloan-Kettering Cancer Center | Fax: (212) 794-4019 1275 York Ave. Box 98 | New York, NY 10021 | ==============================================================================
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