Consecutive washes with bleach/detegent - 50% ethanol - 0.5 M NaCl should remove most of the stuff which attaches to the tubing. Ethanol dissolves many poorly water soluble (hydrophobic) dyes which may attach (via precipitation) to the tubing. Since nearly all dyes are charged molecules, they also attach electrostatically to the tubing. The best way to remove so attached molecules is to rise ionic strength (e.g. 0.5 M NaCl), which breaks ionic bonds. Zbigniew Darzynkiewicz
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