Re: beads

From: Gerhard Nebe-von-Caron (Gerhard.Nebe-von-Caron@unilever.com)
Date: Wed Feb 12 1997 - 08:04:08 EST


          Depending on the purpose of it you might get a way with 
          surface labeling some yourself with FITC, but even that is 
          not only green.

          Otherwise you could try:

          Bangs Laboratory at
          http://www.bangslabs.com/

          Molecular probes at 
          http://www.probes.com/

          prolabo about estapor beads on 
          http://www.prolabo.com/products/esta.htm

          seradyn under
          http://www.seradyn.com/particletech.html


          We use 0.5u coumarin labeled beads (yellow-green) as 
          counting standard for the bacteria. The samples are 
          routinely run in 0.05% tween20 to keep the CV's low. That 
          also reduces carryover and does not effect the cell 
          viability.

          Regards

          Gerhard Nebe-v.Caron
          Unilever Research, Colworth,
          Sharnbrook, Bedfordshire
          GB - MK44 1LQ
          Tel:    +44(0)1234-222066
          FAX:    +44(0)1234-222344
          gerhard.nebe-von-caron@unilever.com     



______________________________ Reply Separator _________________________________
Subject: beads
Author:  agawin@azathoth.ceab.es at INTERNET
Date:    12/02/97 02:44


Hello to all flow-ers:

Thanks to all those who replied on cleaning the machine after passing <2 um
beads. One more question : where can we buy beads that fluoresce ONLY in
green, around 1 um size. We asked Polyscience and they say that these beads
are to be custom made. We would appreciate any info. on where to look for
these. Thanks in advance!

Nona Sheila R. Agawin
Centro de Estudios Avanzados de Blanes (CEAB)
Consejo Superior Investigaciones Cientificas (CSIC)
Cami de Sta. Barbara s/n
17300 Blanes, Girona
Spain
FAX: 34 72 337806
Phone: 34 72 336101
WWW page: http//www.ceab.es



This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:22 EST