Hello, I was wondering if somebody has experience detecting fusionproteins with a "flag" epitope, using a commercial available anti-flag antibody for intracellular expression... I haven't tried it, but would like to know if this could possibly work, especially for somebody who want's to analyse cellcycle expression of a transfected protein using a flag-fusion construct. Any details on which mAb to use, minimal expression levels, staining procedures would be very wellcome. Thank you very much, Matthias _____________________________________________________________________________ Matthias Haury Flowcytometry Dept Immunology Institut Pasteur mhaury@pasteur.fr Tel: 33 (01) 40 61 31 29 Fax: 33 (01) 45 68 86 39 _____________________________________________________________________________
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