I got this address from Dorothy at Medical Sciences Building, University of Alberta, Edmonton. The project invoves using S.C. yeast with a mammalian construct inserted. We would like to confirm the presence of the mammalian construct within the yeast using a FACS scan and antibodies against epitopes iwthin the mammalian construct. Is the procedure for this protocol the same as with mammalian cells with the addition of a 45 second sonication step at the end before running a FACS scan? I appreciate any comments you may have with regards to this question? Yours sincerely, Jihan Marjan Ph.D.
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