Hello all, I've just been approached by someone wishing to look at RNA expression using flow cytometry. He wants to do 2-colour analysis if possible and already has his probes made up. What would be the best approach (We're using a FACScan - 488nm) ? I think his probes are DIG-labelled, so we might try to use an anti-DIG antibody labelled with FITC or PE. Does anyone have any basic protocols or ideas that we might be able to use, as RNA probes and analysis by FACS is a new and exciting approach over here. Thanks in advance Julia Philips ************************************************** Julia Philips Institute of Dental Research 2 Chalmers St Surry Hills NSW 2010 Australia PH: 61-2-9282 0358 FAX: 61-2-9282 0368 email: jphilips@extro.ucc.su.oz.au **************************************************
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