Colleagues, It was recently indicated on this mailing list that a four color combination on the FACSCalibur might be FITC/PE/PE-Cy5 and APC-Cy7. This would be very useful to researchers who simply want to update existing three-color options by adding a fourth color (APC-Cy7) as opposed to the FITC/PE PE-Cy7/ and APC option for fourth color which would essentially demand upgrading from 2 color since both PE-Cy7 and APC would be "new" in so much as a FITC/PE/ PE-Cy5 three color combo has become fairly standard. Based on the filter combination in the Calibur however it seems that the latter color combo is optimal since the PE-Cy7 would pass the 670LP in FL3 and the APC would enter FL4 across the 661/16. How would this work with the former color combination? APC-Cy7 with emission around 780 would be blocked by the 661/16. Is Mario Roederer suggesting switching filters between FL3 and FL4? I've noticed the filters in front of the PMT's to FL1 and FL2 on the Calibur are relatively accessible but the filters to FL3 and FL4 are not? Gene Pizzo/UCONN Health
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