Look it up in the Kabat book of "sequences of proteins of immumological interest" or on the database. This informatioj is readily available. I used to work on this in Grad. school. If you want an exact sequence then mail or phone me and I can get it for you Dr. Fergus Byrne Dept. Medicine UCLA Tel. 310-7947195 fbyrne@med1.medsch.ucla.edu Fergus ------------------------------------------------------------------------------ REPLY FROM: Byrne, Fergus, M.D. Return-Path: <owner-cyto-sendout@flowcyt.cyto.purdue.edu> Received: from dragon.medctr.ucla.edu by deans.medsch.ucla.edu id <32ED4C24@deans.medsch.ucla.edu>; Mon, 27 Jan 97 16:45:24 PST Received: from flowcyt.cyto.purdue.edu (flowcyt.cyto.purdue.edu [128.210.60.31]) by dragon.medctr.ucla.edu (8.8.4/8.8.4) with SMTP id QAA04539; Mon, 27 Jan 1997 16:42:36 -0800 (PST) Received: by flowcyt.cyto.purdue.edu (940816.SGI.8.6.9/930416.SGI.AUTO) for cyto-sendout id SAA10987; Mon, 27 Jan 1997 18:35:51 -0500 Received: from marlin.bio.umass.edu by flowcyt.cyto.purdue.edu via ESMTP (940816.SGI.8.6.9/930416.SGI.AUTO) for <cytometry@flowcyt.cyto.purdue.edu> id MAA09038; Mon, 27 Jan 1997 12:45:42 -0500 Received: from alfred.micro.umass.edu (alfred [128.119.49.168]) by marlin.bio.umass.edu (8.8.4/8.7.3) with SMTP id MAA11503 for <cytometry@flowcyt.cyto.purdue.edu>; Mon, 27 Jan 1997 12:39:41 -0500 (EST) Date: Mon, 27 Jan 1997 12:39:41 -0500 (EST) Message-Id: <199701271739.MAA11503@marlin.bio.umass.edu> X-Sender: edavis@marlin.bio.umass.edu X-Mailer: Windows Eudora Light Version 1.5.2 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> From: Erin Davis <edavis@microbio.umass.edu> Subject: Mouse ab constant regions ------------------------------------------------------------------------------ HI - I'm looking for a primer for a mouse IgG1 (heavy chain) antibody. I have the amino terminal sequenced so I have a 5' primer. Now, I need a primer 3' of the variable region... ... Does anyone know where I can get the sequences of the J regions or the CH1 region? I'd really appreciate it! Thanks!!! Erin E Davis Department of Microbiology University of Massachusetts at Amherst Email: edavis@microbio.umass.edu Voice: 413-545-4220 Fax: 413-545-1578
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