Re: viability + PE-Ab

From: nan harvey (nharvey@fiona.umsmed.edu)
Date: Fri Jan 24 1997 - 22:52:03 EST


Make that a very low concentration of FDA--a little goes a long way!

Nan Harvey
University of Mississippi Medical Center
Jackson, MS


On Fri, 24 Jan 1997, Miller, Mark A. wrote:

> 
> For assesing viability in conjunction with a PE-Ab, use
> fluoroscein diacetate (FDA.)
> 
> FDA is NON-fluorescent, but after entering intact cells it
> is cleaved by non-specific esterases to produce green
> fluorescent fluorscein.
> 



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