In response to Wallace Lauzon, Tue Jan 21 1997 A while back (last year?!) we were doing a quite a bit of work with trypan blue for use as a counterstain. I presented a poster and a talk in Charleston where we demonstrated this technique for use with surface antibodies, in situ PCR, and in situ hybridization. We have successfully used it in single and dual color (FITC/PE) staining. The paper has been submitted, but if you'd rather get the info sooner than later, I'd be more than happy to share our protocol, and experiences with you. Drop me an e-mail. VLM646@nwu.edu Viki Mosiman RHLCC Flow Cytometry Core Research Facility Northwestern Univ
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