Hello, I've been lurking around this E-mail list for about a month now enjoying the discussions and the information being exchanged. I have recently heard of a technique used in flow based in situ PCR where the autofluorescence is greatly reduced by the addition of trypan blue. It was just a casual reference in a talk and I was unable to find anything more about it from the speaker. I was wondering if there was anyone out there with experience in this technique who could provide a protocol or a good reference for one. While I have your ear, I would also appreciate any information that you may have concerning epitope tagging reagents: anti-CDx-DIG/anti-DIG-PE etc. If there are commercially available reagents I would be eager to find out the sources. Thank you for your time Wallace Lauzon Dept. Microbiology and Immunology University of Ottawa 451 Smyth Rd Ottawa, Canada
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:18 EST