We are the reference lab to which all BM samples from suspected and confirmed pediatric ALL patients enrolled in Children's Cancer Group (CCG) clinical trials are sent. In addition, we monitor the status of patients being treated in FDA trials with immunotoxins originating from our program. In other words, we deal with large numbers of BM samples from patients. What we ask for, and what has worked well, is 5 cc of BM aspirate. From that volume, we do flow cytometric immunophenotyping and sorting for enumeration of clonogenic progenitor cells in colony assays. Kevin G. Waddick, Ph.D. Asst. Professor University of Minnesota Biotherapy Institute 2625 Patton Road Roseville, Minnesota 55113 (612) 627-1920 ext. 131 Office (612) 627-1928 Fax On Fri, 17 Jan 1997, Maryalice Stetler-Stevenson wrote: > > Question for Clinical Flow People: > > > What volume of bone marrow do you aspirate for flow. If you aspirate too > much you get blood, not bone marrow. However, you want as much as possible > from a single site. I had thought 1mL per site was maximum but was told > today that 3 mL gives a good yield. Please share you specifications and I > will summarize for those interested. > > > Maryalice > > Maryalice Stetler-Stevenson > Director Flow Cytometry Unit > Laboratory of Pathology, NCI, NIH > >
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