Apoptosis detection with Annexin V and DiOC6

From: Christian Stöckigt (stoeckig@uni-duesseldorf.de)
Date: Fri Jan 17 1997 - 16:19:40 EST


Hi Flow- ers!

We are trying to detect induced apoptosis with Annexin V and DiOC6 staining
followed by FACScan analysis. Until now, our results are not satisfying-
Staining with rhodamine works well.
Does anyone know a working protocol (apart from any datasheet or catalog
information!) and/or has experience with these staining methods? Please
reply directly to my Email

Thanks in advance

Christian Stoeckigt	

Immunology Laboratory
Institute for Hemostasis and Transfusion Medicine
Heinrich- Heine University Medical Center
Duesseldorf, Germany

stoeckig@uni-duesseldorf.de



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