Hi Everybody, We recently started doing intracellular staining for cytokines on a FACScan with CellQuest and are trying to resolve three issues. 1) We see substantial IL-2 and IFN-g positive cells. Is there a rational, operator-independent way of setting the markers so that the number of positives can be identified? Is there software that will do this? 2) There are a few IL-4 positives and I am not sure that I want to trust those low percentages? Is there a better way to detect Th2 cells? 3) Does anybody have experience staining for TNF-beta (or alpha) or IL-10? Thanks in advance for any feedback. Murali Ramanathan Pharmaceutics
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