> >Hello, > >Are Reed-Sternberg/CD30 (ki-1) cells generally found by FCM methodologies? >Are there any tripple staining techniques to specifically select for these >cells? Maybe CD30 FITC with CD5 PE and CD19 CY5-PE ? What are the light >scatter properties Reed-Sternberg cells? We have not had the opportunity to >correlate FCM-CD30 with histological staining. ANY comments would be >appreciated. The literature suggests some expression of CD30 in some >non-Hodgkins lymphomas. > >---------------------------------------------- >Bill Baughman >Americal Medical Laboratories, Inc., FCM >E-mail: fcm@amlimmun.dgsys.com >---------------------------------------------- > >Hi, There are cases of higly malignant T cell NHL and even very rare cases of large cell B-cell NHL (which are not KI-1 NHL by morphology and other markers) that are CD30 positive. Moreover occasional T cells in HD lymph nodes can be CD30+. R-S cells are difficult to get in suspension and FCM methods are of very limited use in diagnosis. We usually do immunostaining on paraffin/frozen sections. Best wishes Anna Porwit-MacDonald Haematopathology, Dept. of Pathology, Karolinska Hospital, Stockholm, Sweden > >
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