For our human progentitor evaluation in blood, marrow, and cord blood we
prepare a 'lineage cocktail' of FITC conjugated monoclonals defining mature
cells Tcell (CD3), Bcells (CD20) Mono/Myeloid (CD14, CD15, C16) in combination
CD34 and other markers of interest for three color analysis; then gating on the
Lin- or Lin- CD34+ population. The negative controls we use are are (1)
autofluoresence and (2) Lin cocktail + (FL2 or FL3)CD34 + (FL2 or FL3) Isotype.
April G. Durett
Technology Transfer - Flow Cytometry
Blood & Marrow Transplantation
Univ Texas MD Anderson Cancer Center
To: cyto-inbox
cc:
From: Arnold_Mixon @ nih.gov (Arnold Mixon) @ INTERNET
Date: 01/15/97 02:55:41 PM
Subject: murine stem cells
An investigator read somewhere(?) of a panel of monoclonal Abs used
to identify murine progenitor/stem cells. Of interest is an antibody called
"lin-neg." Anyone out there heard of it before/know of it's source? It
sounds like it might be a cocktail of different Abs.
Thanks,in advanced, to all responses.
Arnold
Arnold Mixon
National Institutes of Health
N.C.I., Surgery Branch
Flow Cytometry Lab
Tel.# 301-496-9816
E-mail: Arnold_Mixon@nih.gov
"Just the FACS Ma'am - I only want the FACS !!"
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