Hoechst Red fluorescence?

From: Ayala Sharp (lssharp@weizmann.weizmann.ac.il)
Date: Thu Jan 16 1997 - 08:59:16 EST

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A paper in J. Exp. Med. Vol. 183 p. 1797 by Goodell et. al.
describes the isolation of Murine Hematopoietic stem cells
using Hoechst  33342 staining. They excite at 350nm and
measure fluorescence at two wavelengths using a 450 BP
(450/20) and 675 EFLP filters with a 610 dichroic mirror.
They show a nice plot of Hoechst Red versus Hoechst Blue.
The literature I've seen shows no Hoechst fluorescence in
the red region. Any comments ? Explanations ? Suggestions ?
         Thanks,  Ayala.

-------------------------------------------------------------------
Dr. Ayala Sharp
Biological Services,
Weizmann Institute of Science,
Rehovot, ISRAEL.
FAX: 972-8-9344113
E-mail: lssharp@weizmann.weizmann.ac.il

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