I always found it to difficult to compensate PI out of the
orange channel. I don't know if 7AAD DNA profiles are to
lousy, but I always wondered if there is a possibility of
just reducing the PI intensity by mixing the PI with a
competing molecule that does either is just not fluorescent
quenches of causes energy transfer. Perhaps Howard could
shed some light on that.
Gerhard Nebe-v.Caron
Unilever Research, Colworth,
Sharnbrook, Bedfordshire
GB - MK44 1LQ
Tel: +44(0)1234-222066
FAX: +44(0)1234-222344
gerhard.nebe-von-caron@unilever.com
______________________________ Reply Separator _________________________________
Subject: Two antigens plus DNA staining
Author: ratech@aecom.yu.edu at INTERNET
Date: 15/01/97 03:10
Hi Flowers:
We have a project in which we would like to study DNA ploidy plus protein
expression of two nuclear antigens. Our intrumentation is limited to a
FACSCAN. I assume that we will start by using FITC and propidium idodide.
But what, if any, is your recommendation for a third color? Is this
feasible with the limitation of our instrument?
Thanks.
Howard Ratech, MD
Albert Einstein College of Medicine/
Montefiore Medical Center
Bronx, New York
ratech@aecom.yu.edu
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