Hi flowers, I´m interested in detection/quantification of cytoplasmatic and/or intranuclear STAT proteins by fluorescence microscopy or flow. Has anybody experience with these proteins? E.g.: - Is the amount of protein/cell above the detection limit? - Are fixation and staining protocol(s) available? All papers I found so far describe use of anti-STAT mAbs for Western blotting analysis etc. but not for immunofluoescence stainings. Every suggestion is welcome! Thanks in advance Georg ------------------------------------------------------------------------ H.G. Kreysch Merck KGaA Biomed Fo/IMO - A25/410 Tel.: (49)6151 - 72 7323 Frankfurter Str. 250 FAX: (49)6151 - 72 7398 D-64271 Darmstadt E-Mail: kreysch@merck.de
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