Dear Flowers, We would like to try and set up a method of flow cytometric analysis for Collagen Type III on bone marrow stromal cells, has anyone tried this before using the flow cytometer? The primary antibody that we have in the lab is Goat anti-Type III Collagen (Southern Biotechnology Associates, Inc) and we have a rabbit anti-goat IgG FITC conjugate (Sigma F-7367) as a secondary antibody. We would have to purchase an isotype matched negative control. Any ideas on applying these reagents to the flow would be most welcome. Thankyou in advance Denise Janssens Haem Lab Med School Cape Town S.A e-mail : dj@samiot.uct.ac.za
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