Clone 4B2 can be obtained from the ATTC for a nominal fee. This pan CD45 hybridoma is easy to grow and secretes an IgG2 antibody that can readily be purified on Protein A sepharose. It immunoprecipitates well and should also make a good immunoaffinity column (use cross-linked protocols for e.g. see J Biol Chem 57: 10766-10769, 1982, or J Biol Chem 261: 2418-2424, 1986). We have also conjugated this antibody woth FITC and PE:Cy5 with good success and these conjugates work very well in ISHAGE-style CD34 cell enumeration protocols (Exp. Hematol. 22: 1003-1010, 1994). Hope this is helpful, D. Robert Sutherland, University of Toronto, and Toronto Hospital
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