Does anyone have experience in the detection of gene products involved in cell cycle control eg cyclins, cdks, p21, p27 etc in cell suspensions. I am planning to measure such proteins by indirect immunofluorescence concurrently with DNA content using either PI or Hoechst staining in HeLa cells. There is an extensive literature on changes in expression as detected by Western blotting, there are immunofluorescence micrographs, but I have not found information on flow cytometry. It seems an obvious approach, can anyone direct me to a reference?
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