DNA + 3-color surface staining

From: Steve Neben (SNeben@genetics.com)
Date: Thu Jan 09 1997 - 14:31:23 EST

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Dear Flow-ers:

I'm looking for a good method for staining bone marrow cells for
simultaneous cell cycle analysis and 3-color surface
immunofluorescence. Antibodies are FITC-, PE-, and biotin-conjugated.
I'm using a FACStarPlus with Argon, He-Ne, and UV lasers. Can anyone
suggest a good DNA stain and avidin conjugate, and staining method
that will work for this configuration ?

Steve Neben
Genetics Institute
Cambridge, MA

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