BMS 631 - 3 Credit Course Lectures: Spring
BMS 632 - 3 Credit Course Practical: Summer
(Lecture course is a prerequisite for practical)
Ideal for Graduate Students, Seniors - Faculty & Staff welcome to sit in
Course Description
BMS 631: Flow Cytometry - Theory.
A thorough excursion into the theory of flow cytometry. This course seeks to convey what the technology is, how it operates and where the applications exist. The course will cover optical systems, including filters, light excitation and emission, fluorescent dyes and fluorescent molecules, electronics including detection systems, signal processing, data analysis and computer applications. Lasers and other light sources as well as quality control will be discussed. Finally, an overview of the application areas in medical science, biological research, and other possible areas of use will be presented. There are NO PREREQUISITES.
BMS 632: Flow Cytometry - Practical Laboratory
A continuation course from the theory of flow cytometry, this course will involve a practical application of the technology. Students will be expected to be involved in a significant research project in which they learn the practical operation of a flow cytometer and produce their own data. It will be necessary for students to have a thorough understanding of the theory of flow cytometry before beginning this course. Aspects of quality control, adequate standards and references and an understanding of data analysis will be part of this course. PREREQUISITE: BMS 631.
At the end of the courses, all participants should be capable of understanding the theory and should be capable of operating instrumentation currently housed in the Cytometry Laboratories at Purdue University.
The courses are STRICTLY LIMITED because of the need for practical participation. Acceptance into the course will be at the instructor's discretion; however, current graduate students will be given priority.
Course Structure
Instructor: J. Paul Robinson, Phone 4-0757; Department of Basic Medical Sciences, School of Veterinary Medicine. (email the course instructor for additional information)
BMS 631 - Lecture course : Tues, Thurs 3:30-5:00 (Dr. J. Paul Robinson unless otherwise indicated).
Page numbers are from H.M. Shapiro, Practical Flow Cytometry, Wiley-Liss, 1994
| Lecture 1 | Background and History | P 43-56 |
| Lecture 2 | The who's and why's of flow cytometry | p 57-72 |
| Lecture 3 | Light and matter | p 75-93 |
| Lecture 4 | Optical Systems | p 93-97 |
| Lecture 5 | Light Sources | p 97-115 |
| Lecture 6 | Light Collection | p 116-126 |
| Lecture 7 | Detectors | p 127-133 |
| Lecture 8 | Flow Systems | p 133-143 |
| Lecture 9 | Electronic measurements | p 145-149 |
| Lecture 10 | Signal Processing | p 149-162 |
| Lecture 11 | Linear, Log and Ratios | p 163-171 |
| Lecture 12 | Precision Sensitivity and Accuracy | p171-177 |
| Lecture 13 | Data Analysis | p179-198 |
| Lecture 14 | Frequency Distributions | p 199-207 |
| Lecture 15 | Multiparameter Data Analysis (MDA) | p 207-214 |
| Lecture 16 | MDA con't ….Compensation | p 207-214 |
| Lecture 17 | Sorting | p 217-228 |
| Lecture 18 | Parameters and Probes I (P&P) | p 229-242 |
| Lecture 19 | P&P II - Intrinsic Parameters | p 242-251;274- 288 |
| Lecture 20 | Applications: Cell Cycle & DNA analysis I | p 251-272; 388-392 |
| Lecture 21 | Applications: Cell Cycle & DNA analysis II | p 251-272; 388-392 |
| Lecture 22 | Immunology | p 289-296; 393-401 |
| Lecture 23 | Functional Studies | p 306-347 |
| Lecture 24 | Food Science, Microbiology | p 342; 412-425 |
J.Paul Robinson, PhD PH:(765)4940757 Professor of Immunopharmacology Purdue University FAX:(765)4940517 EMAIL:jpr@flowcyt.cyto.purdue.edu WEB: http://www.cyto.purdue.edu
BMS 632 - Lab/practical: Summer session, time to be arranged
(Lecture course is a prerequisite for lab course)
