BMS 524, 1 Credit Course
Lectures: Spring semester
BMS 527, 1 Credit Course
Practicals: Maymester
Professor: J.Paul Robinson (email the course instructor for additional information)
Ideal for Graduate Students, Seniors - Faculty & Staff invited to monitor/audit course (note an average of 3-5 staff monitor/audit this course each spring)
Course Description: (Previously offered Spring 1995, 1996, 1997, 1998)
Confocal Microscopy provides a powerful tool for evaluation of the 3D structure of
a variety of tissues and biological
samples, as well as some non-biological samples. In addition, using the
multiple excitation capacity of these instruments
it is possible to identify multiple sites of interest
within a single sample and to reproduce these data using image
reconstruction techniques. Live cells can also be evaluated
using these technologies to monitor calcium fluxes, pH
changes or production of oxygen and nitrogen radicals using fluorescent probes.
Participants will become familiar with the rudiments of confocal and
fluorescent microcsopes, and associated
technologies. They will learn the fundamentals of fluorescence detection
and develop a keen understanding of the use of
and availability of a variety of fluorescent probes across the UV and
visible spectrum. They will also be introduced to
basic image analysis techniques.
Participants will learn to use both the UV/visible confocal scope and the
associated operating software. They will have
the opportunity to run the instrument in small groups and will be required
to produce images demonstrating their
capability. They will also learn the use of the real-time linescan confocal
scope using video acquisition software
including Universal Imaging Metamorph software.
At the end of the course, all participants should be capable of understanding
the theory and should be capable of
essentially operating instrumentation currently housed in the Cytometry Laboratories
at Purdue University.
The practical course (BMS 527) is STRICTLY LIMITED because of the need for microscope time.
Acceptance into the course will be at the instructor’s discretion; however, current graduate students will be given priority. There are NO PREREQUISITES.
Course Structure (BMS 524):
Instructor: J. Paul Robinson
Lectures: 16 hours (Spring semester)
Practical Demonstrations (BMS 527): 18 hours (Maymester)
Practical Participation (BMS 527): 28 hours (Maymester)
Tentative Schedule (may change): Lectures will be Wednesdays 9:30 - 10:20 during Spring semester. Practicals will be given during Maymester: practical demonstrations will be Tuesday 1-3 pm; Thursday 1-3pm; practical (hands-on) sessions will be Tuesday and Thursday 3-5 pm.
This course is offered through the Purdue University Cytometry Laboratories which is
under the Department of Basic Medical Sciences in the School of Veterinary Medicine. Funding for the Course is provided by the Department, the Vet School, and via the Vice President's Reinvestment Fund.
Lectures:
(Given by Dr.J. Paul Robinson unless otherwise indicated)
1. Introduction to Microscopy (part 1)
2. Introduction to Microscopy (part 2)
3. Fluorescence and Fluorescent Probes
4. The Principles of Confocal Microscopy
5. Introduction to the Bio-Rad MRC 1024 Confocal System
6. Sample Preparation for Confocal Microscopy
7. Image Formats and Image Manipulations
8. Principles of 2D Image Analysis - Morphometry(I) (Dr. John Turek)
9. Applications of Morphometry (II) (Dr. John Turek)
10. 3D Reconstruction and Visualization
11. Confocal Microscopy for Neuroscience (Dr. Terry Powley)
12. Live Cell Applications
13. Calcium Imaging Applications (Dr. Ken Robinson)
14. Stereology Approaches to Image Analysis (Dr. Gert Breur)
15. Advanced Applications of Imaging: 2 Photon Imaging (TBA)
16. Course Overview
Grading: BMS 524 - Final Examination: 100%
Practicals (BMS 527) - preparation & analysis of appropriate images: 100%
