Confocal Microscopy Instrumentation
  • Some small problems we have encountered and the solutions.

  • How we use the heated stage for physiology measurements

    • Bio-Rad MRC 1024 UV/Vis System

    The MRC 1024 installed in the lab consists of the following components:

    Lasers: Microscope:
    • Nikon Diaphot 300 (Inverted)
    • Objectives:
      • Plan APO 10 x 0.45
      • Fluor Phase 10 x
      • Flour 20 x 0.75
      • Fluor 40 x 1.3 DIC OIL
      • Plan APO 60 x 1.4 DIC OIL
      • Plan APO 100 x 1.4 OIL

    • DIC Condenser and optical components
    • EPI - Fluor Attachment with 100 w HG lamp, Powersupply for HBO 100w
    • Stage:
    • Z-Axis:
      • Computerized Z-Motor model #31002 plus controller
    • Vibration Table
      • TMC Model 78-455-12 Size: 8' x 4', drilled 3/16 plate every 1"
      Physiology Components:
      • Heated Stage: DTC-W-120-LC Digital Temperature Controller & Stage Brook Industries made to order (see Picture)
      • Objective Heater: OH-100 Objective Heater Brook Industries made to order
    MRC-1024 Options:

    Computer System:
    • Compaq Prosignia 500 with 80 Meg ram, 21" Monitor
    Networking:
    • Novell 4.01

    Archival Storage/Backup:
    • Philips CD Rom Writer
    • Corel Optical disk
    Output/Presentation:
    • Printers
    • Codonics NP1600 Dye Sublimation Color Printer
    • H-P 4M Laser Printer
    • Tektronics Color Laser Printer
    Digital Camera
    • Lasergraphics LFR Personal
    Workstations:
    • 2 x Silicon Graphics Workstation (Indigo 2) (see data analysis above)
    Bio-Rad DVC 250 Viewscan
    The DVC 250 installed in the lab consists of the following components:
    Lasers:
    • Visible: ILT Ar-Kr Model 5470K-00C-2B
    Microscopes:
    • Nikon Diaphot 200 (Inverted) with computerized Z-Motor model #31002 plus controller
    • Nikon Labophot (Upright) with computerized Z-Motor model #31002 plus controller
    Camera:
    • Monochrome: Photonic Sciences "Darkstar"
    • Color: Optronics 470T
    Computer:
    • Compaq Prosignia 500 with 80 Meg ram, 21" Monitor
    Image Analysis Software:
    • Metamorph/Metafluor, Universal Imaging Corp.
    Video Monitor:
    • Sony HR Trinitron
    Filter Wheel:
    • Lambda 10-2, Suter Instrument Company
    Video Laserdisc:
    • Panasonic TQ-3031F Laser video disc
    Physiology Components:
    • Heated Stage: DTC-W-120-LC Digital Temperature Controller & Stage Brook Industries made to order (see Picture)
    • Objective Heater: OH-100 Objective Heater Brook Industries made to order