Chapter 1

Myron R. Melamed

-- Cytometry. Historical Perspective

Alice L. Givan

-- Principles of Flow Cytometry: an Overview

Louis A. Kamentsky.

-- Laser Scanning Cytometry

J. Paul Robinson

-- Principles of Confocal Microscopy

Howard M. Shapiro

-- Optical Measurements in Cytometry: Light Scattering, Extinction, Absorption and Fluorescence.

Harry A. Crissman and John A. Stainkamp

-- Flow Cytometric Fluorescence Lifetime Measurements

Howard M. Shapiro

-- Principles of Data Acquisition and Display

Larry Seamer and Larry A. Sklar

-- Time as a Flow Cytometric Parameter

Kevin L. Holmes and Larry M. Lantz

-- Protein Labeling with Fluorescent Probes

J. Philip McCoy, Jr

-- Preparation of Cells from Blood

Carleton C. Stewart and Sigrid J. Stewart

-- Cell Preparation for Identification of Leukocytes

Steven K. Koester and Wade E Bolton

-- Strategies for Cell Permeabilization and Fixation in Detecting Surface and Intracellular Antigens

James W. Jacobberger

-- Stoichiometry of Immunochemical Staining Reactions and Flow Cytometry of Intracellular Antigens

Robert A. Hoffman

-- Standardization and Quantitation in Flow Cytometry

Gloria Juan, Frank Traganos and Zbigniew Darzynkiewicz

-- Identification of Mitotic Cells by Flow Cytometry

Nicholas H.A. Terry and R. Allen White

-- Cell Cycle Kinetics Estimated by Analysis of BrdUrd Incorporation

A. Bruce Lyons, Jhagvaral Hasbold and Philip D. Hodgkin

-- Flow Cytometric Analysis of Cell Division History Using Dilution of CFSE, as Stably Integrated Fluorescent Probe

Elmar Endl, Christiane Hollman and Johannes Gerdes

-- Antibodies against the Ki-67 Protein: Assessment of the Growth Fraction and Tools for Cell Cycle Analysis

Jorgen K. Larsen, Göran Landberg and Göran Roos

-- Detection of Proliferating Cell Nuclear Antigen (PCNA)

Andrea Fattorossi, Alessandra Battaglia and Cristiano Ferlini

-- Lymphocyte Activation Associated Antigens

Andrea Cossarizza and Stefano Salvioli

-- Analysis of Mitochondria during Cell Death

Steven K. Koester and Wade E. Bolton

-- Cytometry of Caspases

Iona E. Weir

-- Analysis of Apoptosis in Plant Cells

Zbigniew Darzynkiewicz, Elzbieta Bedner and Frank Traganos

-- Analysis of Apoptosis: Common Pitfalls and Misconceptions

Nicole Dodger Zantek and Michael S. Kinch

-- Analysis of Cell Migration

Sherry L. Voytik-Harbin

-- 3D Extracellular Matrix Substrates for Cell Culture

5. S.L. Voytik-Harbin, B. Rajwa and J.P. Robinson

-- 3D imaging of Extracellular Matrix and Extracellular Matrix-Cell Interactions

Michael S. Kinch

-- Cytometric Analysis of Cell Contact and Adhesion

Emma T. Bowden, Peter J. Coopman and Susette C. Mueller

-- Invadopodia: Unique Methods for Measurement of Extracellular Matrix Degradation in vitro

Jaroslav Dole_el, Martin A. Lysák, Marie Kubálková, Hana _imková

-- Sorting of Plant Chromosomes

Heinz-Ulli G. Weier

-- Quantitative DNA Fiber Mapping

Johnny Hindkjær and Steen Kølvraa

-- Primed IN Situ Labeling (PRINS)

Steven S.S.Poon and Peter M. Lansdorp

-- Measurements of Telomere Length on Individual Chromosomes by Image Cytometry

Jingly Fung, Santiago Munne and Heinz-Ulli G. Weier

-- Detection of Chromosome Translocation Products in Single Interphase Cell Nuclei

Martin Poot and Robert H. Pierce

-- Analysis of Mitochondria by Flow Cytometry

Peter Østrup Jensen, Jacob Larsen and Jörgen K. Larsen

-- Analysis of RNA Synthesis by Cytometry

Ralph M. Bohmer

-- Flow Cytometry of Erythropoiesis in Culture:Bivariate Profiles of Fetal and Adult Hemoglobin

Luca Pierelli, Giovanni Scambia and Andrea Fattorossi

-- Flow Cytometric Analysis of Human Hemopoietic Progenitor Differentiation by Assessing Cell Division Rate and Phenotypic Profile

Paul J. Smith and Marie Wiltshire

-- Cytometry of Antitumor Drug-Induced Target Interactions

Awtar Krishan

-- Monitoring of Cellular Resistance to Cancer Chemotherapy : Drug Retention and Efflux

Ralph E. Durand and Peggy L. Olive

-- Resistance of Tumor Cells to Chemo- and Radiotherapy Modulated by Three-dimentional Architecture of Solid Tumors and Spheroids.

Peggy L. Olive

-- Analysis of DNA Damage in Individual Cells

William D. Wright, Isabelle Lagroye, Peng Zhang, Robert S. Malyapa and Joseph L. Roti Roti.

-- Cytometric Methods to Analyze Ionizing-Radiation Effects

Robert P. VanderWaal, Ryuji Higashikubo, Mai Xu, Douglas R. Spitz, William D. Wright and Joseph L. Roti Roti.

-- Cytometric Methods to Analyze Thermal Effects

Carleton C. Stewart and Sigrid J. Stewart

-- Multiparameter Data Acquisition and Analysis of Leukocytes by Flow Cytometry

Richard J. Clatch

-- Immunophenotyping of Hematological Malignancies by Laser Scanning Cytometry

J-P Vial and Francis Lacombe

-- Immunophenotyping of Acuta Leukemia. Utility of CD 45 for Blast Cell Identification.

Rosella Silvestrini, Aurora Costa and Maria Grazia Daidone

-- Cell Proliferation Markers in Human Solid Tumors: Assessing Their Impact in Clinical Oncology

Andrzej Deptala and Sharon P. Mayer

-- Detection of Minimal Residual Disease

Wojciech Gorczyca, Andrzej Deptala, Elzbieta Bedner, Xun Li, Myron R. Melamed and Zbigniew Darzynkiewicz

-- Analysis of Human Tumors by Laser Scanning Cytometry.

David A. Rew

-- Laser Cytometry of Human Tissues and Tumors: the Clinical Perspective

Thomas W. Mc Closkey

-- Flow Cytometry for Evaluation and Investigation of HIV Infection

Gunter Valet, Luc Kestens, Erika Braeutigam and Friedrich Otto

-- Prediction and Precise Diagnosis of Diseases by Pattern Analysis in Multiparameter Cytometry

Stephanie A. Sincock and J. Paul Robinson

-- Flow Cytometry Analysis of Microorganisms

Harald B. Steen

-- Staining and Measurement of DNA in Bacteria

Mette Walberg and Harald B. Steen

-- Flow Cytometric Monitoring of Bacterial Susceptibility to Antibiotics