European Working Group on
Clinical Cell Analysis (EWGCCA)

Collaboration in the European Community

EU Concerted Action BMH4-CT97-2611(DG12-SSMI)

1. Purpose:

The European Working Group for Clinical Cell Analysis (EWGCCA) was founded in January 1996 in Regensburg, Germany. It had its first regular meeting on July 4, 1996 in Antwerp, Belgium. The speaker of the group is G.Schmitz, Regensburg, Germany with J.Gratama, Rotterdam as treasurer.

The objectives of the group are the advancement and standardization of clinical cell analysis in the European Community. The Working Group promotes the collaboration of various scientific disciplines and societies in this field without the intention to establish a formal scientific society.

2. Membership:

The members of the working group (WM) elect active members (AM) and industrial members (IM).

3. Task Forces:

WM, AM and IM form task forces for specific issues in the field of clinical cell analysis. The following task forces for clinical cytometry have been established:

I. Technical Task Forces:
1. Flow cytometer set-up and calibration (see Concepts in Clinical Cytometry)
Boussu, Rotterdam, Stockholm, Warsaw

2. Sample preparation for multicolor flow cytometry
Aarau, Salamanca, Urbino

3. Absolute cell count assessment
Milano, London, Paris, Stockholm

4. Detection of intracellular antigens
Rotterdam, Athens, Debrecen, London, Paris, Regensburg, Salamanca, Urbino

5. Quantitation of antigen expression
Stockholm, Athens, Boussu, Regensburg

6. Cytometric data analysis (see Concepts in Clinical Cytometry)
Martinsried, Regensburg, Rotterdam

II. Clinical Task Forces
7. Lymphocyte subset enumeration and function assays
London, Boussu, Paris, Regensburg, Stockholm, Urbino

8. Platelet Immunophenotyping and function assays (see Concepts in Clinical Cytometry)
Regensburg, Debrecen, Stockholm, Warsaw

9. Leukemia and lymphoma Immunophenotyping (see Leukemias and Lymphoma Classification)
Salamanca, Athens, Martinsried, Paris, Warsaw

10. CD34+ stem cell enumeration
Urbino, Aarau, Herlev, London, Milano, Rotterdam

4. Consensus Reports:

Consensus protocols for the:
- Immunophenotyping of Hematopoietic Malignancies (see Leukemia 10,877-895(1996))
- Immunophenotyping of platelet dysfunctions (see Concepts in Clinical Cytometry)
- Immunophenotyping of broncheo-alveolar lavage (BAL) samples
- Dual parameter DNA/antibody flow cytometry
have either been elaborated or are currently under work.

4. Working Group Members (WM):

Speaker:
Prof.Dr.Gerd Schmitz
Inst.Clin.Chem.and Lab.Medicine
Franz-Josef-Strauss-Allee 11
D-93053 Regensburg
Germany
Tel: +49/941/944-6200
Fax: +49/941/044-6202
E-mail: gerd.schmitz@klinik.uni-regensburg.de

Treasurer:
Dr.Jan W. Gratama
Dep.Clinical and Tumor Immunology
Dr.Daniel den Hoed Kliniek
Groene Hilledijk 301
NL-3008 AE Rotterdam
The Netherlands
Tel: +31/10/4391-418
Fax: +31/10/4841-885
E-mail: gratama@immh.azr.nl

Other Members:
Prof.Brigitte Autran
CNRS URA 625
Hôpital Pitie-Salpetrière
83, bld de l'hôpital - Bâtiment CERVI
F-75013 Paris
France
Tel: +33/1/4217-7403
Fax: +33/1/4217-7490
E-mail: brigitte.autran@psl.ap-hop-paris.fr

Dr.Bruno Brando
Renal Transplant Unit
Piazza Ospedale Maggiore, 3
I-20162 Milano
Italy
Tel: +39/02/6444-2765
Fax: +39/02/6444-2909
E-mail: bruno.brando@iol.it
Internet: http://wwwamb.casaccia.enea.it/GIC/EWGCCA.html

Ing.Jean-Luc D'Hautcourt
Centre Hôspitalier Régional
Hôpital de Warquignies
Rue de chaufours, 27
B-7300 Boussu
Belgium
Tel: +32/65/786531 ext. 439
Fax: +32/65/778421
E-mail: dhautcou@mail.interpac.be

Prof.Dr.Andreas R. Huber
Dep.Lab.Medicine
Kantonspital Aarau
Buchserstr.1
CH-5001 Aarau
Switzerland
Tel: +41/62/838-5302
Fax: +41/62/838-5399
E-mail: --

Prof.George Janossy
Dep.Clin.Immunology
Royal Free Hospital School of Medicine
Pond Street, Hampstead
London NW3 2QG
UK
Tel: +44/71/431-0879, +44/71/794-0500 ext.3741
Fax: +44/71/431-0879
E-mail: janossy@rfhsm.ac.uk

Dr.Hans E.Johnsen
Dep.Hematology
Herlev Hospital Univ.Copenhagen
Herlev Ringvej 75
DK-2730 Copenhagen
Denmark
Tel: +45/4453-5330, -3924
Fax: +45/4453-2518
E-mail: --

Dr.János Kappelmayer
Dep.Clin.Chemistry
University Medical School
Nagyerdei krt.98
H-4012 DOTE Debrecen
Hungary
Tel: +36/52/431-956
Fax: +36/52/417-631
E-mail: kappelmayer@kki.dote.hu

Dr.Rodica Lenkei
CALAB Medical Laboratory
Katarinavaegen 15
S-10465 Stockholm Laen
Sweden
Tel: +46/8/6159500
Fax: +46/8/6427007
E-mail: rodica.lenkei@calab.se
Internet: http://www.calab.com

Prof.Alberto Orfao de Matos
Servicio General de Citometria
Hospital Universitario de Salamanca
Paseo San Vincente s/n
E-37007 Salamanca
Spain
Tel: +34/23/291375
Fax: +34/23/294624
E-mail: orfao@gugu.usal.es

Prof.Stefano Papa
Centro di Citometria
s/o Istituto di Istologia e Analisi di Laboratorio
University of Urbino
Via Zeppi
I-61029 Urbino
Italy
Tel: +39/0722/327893
Fax: +39/0722/329655
E-mail: citometria@bio.uniurb.it
Internet: http://www.abanet.it/imagitronica/ewgcca

Prof.M.Papamichail
Dep.Immunology
St.Savas Cancer Hospital
Alexandras Ave 171
GR-11522 Athens
Greece
Tel: +30/1/6430083
Fax: +30/1/6420146, 6421022
E-mail: michael@netor.gr

Prof.Thomas H.Totterman
Dept.Clin.Immunol
University Hospital
Uppsala Län
S-751 85 Stockholm
Sweden
Tel: +46/18/664184
Fax: +46/18/507866
E-mail: thomas.totterman@klinimm.uu.se

Prof.Günter K.Valet
AG Zellbiochemie
Max-Planck-Inst.f.Biochemie
Am Klopferspitz 18a
D-82152 Martinsried
Germany
Tel: +49/89/8578-2518, -2525
Fax: +49/89/8578-2563
E-mail: valet@biochem.mpg.de
Internet: http://www.biochem.mpg.de/valet/cellbio.html

Prof.Barbara Zupanska
Inst.Hematology and Blood Transfusion
Chocimska Street 5
PL-00-957 Warsaw
Poland
Tel: +48/22/495742
Fax: +48/22/488970
E-mail: --

For problems or comments, please contact:
G.Valet E-mail: valet@biochem.mpg.de, Max-Planck-Institut für Biochemie, Am Klopferspitz 18a, D-82152 Martinsried, Germany, Tel: +49/89/8578-2518, -2525, Fax: +49/89/8578-2563, INTERNET address: http://www.biochem.mpg.de/valet/cytorel.html
Last update: Dec.14, 1998