Deutsche Gesellschaft für Zytometrie (DGfZ)

DGfZ Abstracts

=== KEY NOTE LECTURES ===

1. MULTIWAVELENGTH 3D MAGING OF IMMUNO- LOGICAL AND GENETICAL MARKERS IN THE CELL NUCLEUS BY CONFOCAL MICROSCOPY AND IMAGE RECONSTRUCTION
M.Robert-Nicoud
Dynamique de l'Organisation du Genome, "DyOGen", Institut Albert Bonniot, Universite Joseph Fourier, 38706 La Tronche Cedex, France.

2. 3D RECONSTRUCTION AND IMAGE ANALYSIS TECHNIQUES FOR CONFOCAL LASER SCANNING MICROSCOPY
Y.Usson and M.Robert-Nicoud
DyOGen, Institut Albert Bonniot, Univ. Joseph Fourier, Grenoble, France

3. HUMAN B CELL MALIGNANCIES: CYTOMETRIC ANALYSIS IN THE CONTEXT OF NORMAL B CELL DIFFERENTIATION
Max D. Cooper
University of Alabama at Birmingham and the Howard Hughes Medical Institute, Birmingham, AL 35294, USA

=== ORAL PRESENTATIONS ===

5. MONITORING OF CELL STATE DEPENDENT PERFORMANCE OF MICROBIAL POPULATIONS IN BIOREACTORS
Th.Bley, S.Müller, A.Lösche
University of Leipzig, Department of Biotechnology, D-04318 Leipzig

6. HIV-RELATED KAPOSI SARCOMA (KS): IMMUNOBIOLOGICAL EFFECTS OF INTERFERON-a (IFNa) AS DETECTED BY FLOW CYTOMETRIC ANALYSIS
I.Böhm, R.Bauer
Department of Dermatology, University of Bonn, Germany

7. EXPERIMENTAL EVIDENCE THAT METHOTREXATE BLOCKS THE BINDING OF INTERLEUKIN 1b TO THE INTERLEUKIN 1 RECEPTOR ON TARGET CELLS
M.Brody, I.Böhm, R.Bauer Department of Dermatology, University of Bonn, Germany

8. DNA CONTENT IN COMPARISON WITH INTRAOPERATIVE PLEURAL LAVAGE AS A PROGNOSTIC FACTOR IN LUNG CANCER
J.Buhr1, R.Deisz1, K.H.Berghäuser2, C.Kelm1, T.Zimmermann1, T.Dreyer2
1Dept. of Surgery and 2Institute of Pathology of Justus Liebig University Giessen

9. SUPERRESOLUTION FAR FIELD LIGHT MICROSCOPY: ITS POTENTIAL FOR THE ELUCIDATION OF NUCLEAR STRUCTURE
C. Cremer
Institute of Applied Physics, University of Heidelberg, D-69120 Heidelberg

10. COMPARATIVE ANALYSIS OF CELL DAMAGE BY POLAR DYE EXCLUSION OR ELECTRICAL CELL VOLUME DETERMINATION
Elmar Endl, Pia Steinbach, Jürgen Schärfe, Sonja Fickweiler, Klaus Wörle, Ferdinand Hofstädter
Institut für Pathologie, Universität Regensburg, D-93042 Regensburg, Schärfe System, D-72760 Reutlingen, Germany

11. COMPARISON OF CELL KINETIC PARAMETERS IN TUMORS OF THE UPPER GASTROINTESTINAL TRACT
K.P. Gilbertz, K.-P. *Sendler, A. *Fink, U. & D. van Beuningen
Institut fur Radiobiologie, Akademie des Sanitäts- und Gesundheitswesens der Bundeswehr, München; *Chirurgische Klinik und Poliklinik, Technische Universität München

12. FLOW CYTOMETRIC ANALYSIS OF S-PHASE FRACTION AND PLOIDY IN NODE-NEGATIVE BREAST CANCER
N.Harbeck, E.Schüren, P.Ziffer, L.Pache, M.Schmitt, Ch.Thomssen, F.JAenicke, P. Dettmar*, W.Nathrath* and H.Graeff
Frauenklinik and *Institut für Pathologie, Technische Universität, München.

13. PHARMACOKINETIC ANALYSIS OF HEPARIN USING PROTAMINE-COATED LATEX PARTICLES AND FLOW (CYTOMETRY
J.Harenberg, L.Piazolo, R.Malsch, D.L.Heene
1st Department of Medicine, Faculty of Clinical Medicine Mannheim, University of Heidelberg, D-68167 Mannheim, Germany

14. ANALYSIS OF HEPARIN BINDING TO HUMAN LEUKOCYTES USING A FLUORESCEIN-5-ISOTHIOCYANATE LABELED HEPARIN FRAGMENT
J.Harenberg, R.Malsch, L.Piazolo, D.L. Heene
1st Department of Medicine, Faculty of Clinical Medicine Mannheim, University of Heidelberg, D-68167 Mannheim, Germany

15. CIMS (CONTINUOUS IMMUNO MAGNETIC SORTER) A POWERFUL TECHNIQUE FOR SORTING OF BIOLOGICAL PARTICLES
R.Hartig, M.Hausmann, M.Kraus, C.Cremer
Institute of Applied Physiol, University of Heidelberg, D-69120 Heidelberg, Germany

16. KARYOTYPIC PROGRESSION AND PROGNOSIS IN ORAL CARCINOMA
Hemmer J, Bartkowiak B
University of Ulm, Division of Tumorbiology, D-89070 Ulm, Germany

17. SEMIQUANTITATIVE MORPHOLOGY, IMMUNOHISTOCHEMISTRY, AND FLOW CYTOMETRY OF HUMAN SPERMATOGENESIS
A.Hittmair, H.Rogatsch, H.Feichtinger, A.Hobisch*, R.Bale, G.Mikuz
Departments of Pathology and *Urology, University of Innsbruck, Austria

18. LIPOSOME MEDIATED UPTAKE OF ANTISENSE OLIGONUCLEOTIDES BY CANCER CELLS AS STUDIED BY FLOW CYTOMETRY AND LASER SCANNING MICROSCOPY
S. Höhl, O.Wilhelm, E.Schüren, M.Schmitt, H.Graeff
Frauenklinik der Technischen Universität München

19. COMBINED FLOW CYTOMETRIC IMAGING AND SORTING IN A MACRO FLOW SYSTEM FOR AQUATIC APPLICATIONS
M.Höreth, W.Runge, E.Gloáner, K.Enzensberger, C.Steiner, V.Kachel
Max-PIanck-lnstitut für Biochemie, D-82152 Martinsried, Germany

20. SORTING AND ANALYSIS OF LYMPHOCYTES INVOLVED IN HUMORAL ALLERGIC RESPONSES
J.Irsch, E.Maggi*, A.Ruffilli$, A.Radbruch
Institute for Genetics, University Cologne, D-50931 Cologne, Germany, *Institute for Clinical Immunology, I-50134 Florence, $Int. Institute of Genetics, I-80125 Naples, Italy

21. CELL-CELL CONTACTS OF THE ADHERING TYPE: ULTRASTRUCTURE AND MOLECULAR COMPOSITION
J.Kartenbeck
Division of Cell Biology, German Cancer Research Center, Heidelberg, Germany

22. RADIOSENSITIVITY OF SIGNAL TRANSDUCTION PATHWAYS LEADING TO ACTIVATION OF THE NADPH OXIDASE
W.Kaffenberger, K.-P.Gilbertz, D. van Beuningen
Institute of Radiobiology, FAF Med.Acad., Munich, Germany

23. DIOXIN (2,3,7,8-TETRACHLORODIBENZO-p-DIOXIN) INDUCED SKEWING OF THYMOCYTE DIFFERENTIATION: ANALYSIS OF EXPRESSION OF ADHESION MOLECULES
J.Kremer, Ch.Esser
Medical Institute of Environmental Hygiene, Auf'm Hennekamp 50, 40225 Düsseldorf

24. IMPROVEMENT OF BLADDER TUMOR IDENTIFICATION BY COMBINED FLOW CYTOMETRIC MEASUREMENT OF DNA ANEUPLOIDY AND DISTRIBUTION OF 486 P ANTIGEN
H.Leyh, A.Lehmer, R.Hartung
Department of Urology, Technische Univ. München

25. DNA-PLOIDY PATTERNS IN BENIGN PROSTATIC HYPERPLASIA*
H.Leyh, A.Lehmer, R.Hartung
Department of Urology, Technische Univ. München

26. PLOIDY PATTERNS IN RENAL CELL CARCINOMA
H.Leyh, A.Lehmer, R.Hartung
Department of Urology, Technische Univ. München

27. CONFOCAL THETA MICROSCOPY AND 4Pi-CONFOCAL THETA MICROSCOPY
S.Lindek, E.H.K.Stelzer
European Molecular Biology Laboratory (EMBL), Light Microscopy Group, D-69012 Heidelberg, Germany

28. PLOIDY PATTERNS IN RENAL CELL CARCINOMA
H.Leyh, A.Lehmer, R.Hartung
Department of Urology, Technische Univ. München

29. SEPARATION OF MESOPHYLL AND BUNDLE-SHEATH CHLOROPLASTS FROM PLANTS OF PHOTOSYNTHETIC C4 TYPE BY FLOW CYTOMETRY
A.Meister, E.Pfündel
Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany

30. POSSIBILITIES AND LIMITS OF FEULGEN CYTOPHOTOMETRY IN PARAFFIN-EMBEDDED TISSUE
W. Mellin
Institute of Pathology, University of Magdeburg

31. PROGNOSTIC IMPACT OF PROLIFERATION IN COMPLETELY RESECTED ADENOCARCINOMA OF THE ESOPHAGUS
H.Nekarda1, T.Nakamura3, K.Becker2, A.Hölscher1, J.R.Siewert1
1Chirurgische Klinik and 2Inst.f. Pathologie der TU, D-81675 München, 3Tokyo Women's Medical College, Tokyo, Japan

32. PAS IIIi - A FLOW CYTOMETER FOR ROUTINE IMMUNOLOGY
V.0st
Partec GmbH, D-48161 Münster

33. RESULTS OF THE COLLABORATIVE STUDY 1994 ON FLOW CYTOMETRIC DNA MEASUREMENTS
F.J. Otto
Fachklinik Hornheide, D-48157 Münster

34. ANALYSIS OF THE BINDING OF HEPARIN ON PROTAMINE COATED LATEX PARTICLES USING THE FLOW CYTOMETRY
L.Piazolo, J.Harenberg. R.Malsch, D.L.Heene
1st Department of Medicine, Faculty of Clinical Medicine Mannheim, University of Heidelberg, D-68167 Mannheim, Germany

35. MORPHOMETRY ON CERVICAL SMEARS
M. Ruhnke and W. Kühn
Dept. Gynecol. Obstet., Klinikum Benjamin Franklin, Free University of Berlin, Germany

36. ENHANCING FLUORESCENCE INTENSITY AND DETECTION OF RARE EPITOPES WITH MAGNETOFLUORESCENT LIPOSOMES
A.Scheffold, S.Miltenyi*, A.Radbruch
Institute for Genetics, University of Cologne, 50931 Cologne, *Miltenyi Biotec GmbH, 51429 Bergisch Gladbach, Germany

37. ANALYSIS OF THE BINDING OF HEPARIN ON PROTAMINE COATED LATEX PARTICLES USING THE FLOW CYTOMETRY
L.Piazolo, J.Harenberg. R.Malsch, D.L.Heene
1st Department of Medicine, Faculty of Clinical Medicine Mannheim, University of Heidelberg, D-68167 Mannheim, Germany

38. QUALITY CONTROL IN FLOW CYTOMETRY THROUGH MULTIPARAMETER DATA ANALYSIS
E.Smet
Becton Dickinson Immunocytometry Systems Europe, Erembodegem, Belgium

39. INVASION ANALYSIS OF LUNG TUMOR CELLS IN GEL MATRICES
Strohmaier A.-R., Spring H., Spiess E.
Biomedizinische Strukturforschung, Deutsches Krebsforschungszen- trum, Heidelberg

40. MONOCLONAL ANTIBODIES AGAINST HUMAN CD34+ CELLS ISOLATED FROM PERIPHERAL BLOOD
A.Thiel, A.Radbruch, G. von Hesberg, S. Milteny*
Institute for Genetics, University of Cologne, 50931 Cologne, *Miltenyi Biotec GmbH, 51429 Bergisch Gladbach, Germany

41. QUANTITATIVE ANALYSIS OF ACTIVE TRANSCRIPTION SITES WITHIN INTACT NUCLEOLI: A LASER SCAN MICROSCOPIC ANALYSIS
M.F.Trendelenburg, O.V.Zatsepina*, H.Spring
Biomedical Structure Analysis Group, German Cancer Research Center (DKFZ), Germany *A.N. Belozersky Institute, Moscow State University, Russia

42. QUANTIFICATON OF INTRACELLULAR CATHEPSIN ACTIVITIES IN HUMAN LUNG TUMOR CELLS BY FLOW CYTOMETRY
B.Ulbricht1, E.Spiess1, W.Ebert2
1DKFZ, AG Biomedizinische Strukturanalye, 69120 Heidelberg, 2Thoraxklinik der LVA Baden, 69126 Heidelberg, Germany

43. CHARACTERIZATION AND SIGNAL TRANSDUCTION OF THE HEAD ACTIVATOR RECEPTOR IN NEURAL CELL LINE NH15-CA2
H.Ulrich, A.Tarnok
ZMNH, Center for Molecular Neurobioloay, UKE, 20246 Hamburg, Germany

44. CRITERIA FOR HIGH-RESOLUTION DNA FLOW CYTOMETRY
W.Ulrich
Flow Cytometric Service Center, D-72667 Schlaitdorf

45. DIFFERENT PROGNOSTIC IMPACT OF INVASION-ASSOCIATED PROTEASES, PLOIDY AND S-PHASE FRACTION IN COLORECTAL CANCER PATIENTS
H.Vogelsang, H.Nekarda, W.Schröder, F.Huber, J.R.Siewert
Chirurgische Klinik rechts der Isar der TU München, D-81675 München

46. IN VITRO FLUORESCENCE ANALYSIS OF THE ENDOGENOUS PHOTOSENSITIZER PROTOPORPHYRIN IX
H.Weingandt1, P.Steinbach1, R.Baumgartner2, M.Kriegmair2, F.Hofstädter1, R.Knüchel1
1Institut für Pathologie der Universität Regensburg, D-93042 Regensburg, 2Urologische Klinik und Poliklinik der Universität München, D- D-81366 München

47. DESIGN CRITERIA FOR FLOW CYTOMETRIC IMAGING
J. Wietzorrek, R. Kühn, M. Stadler, V. Kachel
Max-Planck-Institut für Biochemie, D-82152 Martinsried, Germany

48. LOW CYTOMETRIC ANALYSIS OF THE EPIDERMAL GROWTH FACTOR RECEPTOR (EGF-R) ON HUMAN OVARIAN CARCINOMA CELLS
C.Will, A.Schäfer, E.Schüren, S.Schwarze, M.Schmitt, O.Wilhelm, V.Möbus, H. Graeff
Frauenklinik der TU München and Frauenklinik der Universität Ulm.

=== POSTERS ===

50. THE INFLUENCE OF ALCOHOL, TOBACCO CONDENSATE AND SALIVARY GLAND FUNCTION ON THE ORAL MUCOSA OF THE RAT
Ch.Hofele1, J.Zöller1, H.Maier2, J.A.Born3
1University Hospital of Maxillo-Facial Surgery, 2University Hospital of Oto-Rhino-Laryngology 3University Institute of Pathology, University of Heidelberg, D-69120 Heidelberg

51. IDENTIFICATION OF OXYTOCIN RECEPTORS ON BOVINE LUTEAL CELLS
J.Kotwica1, T.Viergutz2, R.Pöhland2, W.Kanitz2
1Polish Academy of Science Olsztyn, Division of Reproductive Endocrinology and Pathology, Poland, 2Research Institute for the Biology of Farm Animals, Department of Reproduction, Dummerstorf, Germany

52. CIRCADIAN VARIATIONS OF LYMPHOCYTE SUBSETS AND EXPRESSION OF CD44, CD11a AND CD2 IN HUMAN PERIPHERAL BLOOD
T.O.Kleine1, R.Hackler1, P.Zofel2, J.Albrecht3
1 Med. Zentrum fur Nervenheilkunde, Funktionsbereich Neurochemie, Universitat Marburg a.d. Lahn, 2 Hochschulrechenzentrum der Universität Marburg, 3Becton Dickinson, Heidelberg.

53. THE OXIDATIVE AND MEMBRANE POTENTIAL RESPONSE OF PBMC TO TRH
B.Löhrke, M.Derno, W.Jentsch, H.-D.Matthes, G.Dietl
Research Institute of Animal Biology, D-18196 Dummerstorf.

54. B CELL ACTIVATION IN THE CEREBROSPINAL FLUID(CSF): COMPARISON OF INTERLEUKIN 2 RECEPTOR EXPRESSION, INTRACELLULAR IMMUNOGLOBULINS AND OLIGOCLONAL BANDS
E.Mix1, R.Lehmitz2, T.O.Kleine3, R.Hackler3, H.MeyerRienecker1
1 Dept. of Neurology, Hosp. Nerv. Dis., 2 Central Laboratory of CSF Diagnostics, Med. Faculty, University of Rostock, 3 Division of Neurochemistry, Med. Center of Neurosci., University of Marburg, Germany

55. CELL CYCLE AND PHB-SYNTHESIS OF METHANOL UTILIZING BACTERIA UNDER LIMITING CONDITIONS
S.Müller, J.U.Ackermann, A.Lösche, W.Babel, Th.Bley
University of Leipzig, Department of Biotechnology, D-04318 Leipzig

56. SIGNAL TRANSDUCTION THROUGH INTEGRINS IN HEPATOCYTES
B.Nebe1, J.Rychly1, W.Bohn2
1 Department of Internal Medicine, Unversity of Rostock, 2 Heinrich-Pette-Institute at the University of Hamburg

57. FLOW CYTOMETRIC MEASUREMENTS OF SPONTANEOUS APOPTOTIC CELLS IN FRESH TUMOR SAMPLES
K.Romanakis1, A.Genzlinger1, P.K.Gadhia2, H.Zankl1
1 University of Kaiserslautern, Dept. Human Biology and Human Genetics, D-67663 Kaiserslautern, Germany, 2 Dept.Biosciences, South Gujarat University, Surat 395 007, India

58. DNA ANALYSIS BY FLOW CYTOMETRY OF UP TO NINE YEAR OLD METHANOL/ACETIC ACID FIXED CHILDHOOD ALL SAMLPES
K.Romanakis1, A.Argyriou-Tirita2, H.Zankl1, O.A.Haas2
1 University of Kaiserslautern, Dept. Human Biology and Human Genetics, D-67663 Kaiserslautern, Germany, 2 Children's Cancer Research Institute, St. Anna Children's Hospital, A-1090 Vienna. Austria

59. RAPID DENSITOMETRIC AND MORPHOMETRIC IMAGE ANALYSIS ON FROZEN SECTION BY SELECTIVE FLUOROCHROMIC NUCLEAR STAINING
W.Schöll, P.Pürstner, H.Auner
Dept. of Ob/Gyn, Univ. of Graz, Austria

60. HIGHER INDUCTION OF MICRONUCLEI BY MITOMYCIN C AND g-RAYS IN DIFFERENTIATED SOMATIC CELLS THAN IN UNDIFFERENTIATED EMBRYONIC CELLS OF THE MOUSE IN VITRO MEASURED BY FLOW CYTOMETRY
U.Sehlmeyer1, W.Beisker2, A.M.Wobus1
1 Institut für Pflanzengenetik und Kulturpflanzenforschung, D- 06466 Gatersleben, 2 GSF-lnstitut für Biophysikalische Strahlenforschung, D-85764 Oberschleißheim, Germany

61. THE EFFECT OF THE ANTITUMOR PLANT ALKALOID THALIBLASTIN ON THE CELL-CYCLE PROGRESSION OF OVARIAN TUMOR CELL LINES SENSITIVE AND RESISTANT TO CISPLATIN
F.Seifert1, K.-J.Hutter2, D.Todorov3, W.J Zeller1
1 Institute of Toxicology and Chemotherapy, and 2 Institute of Radiology and Pathophysiology, Applied Immunology, Flow Cytometry, 3 National Oncological Center, BG-1156 Sofia, Bulgaria

62. FLOW-CYTOMETRIC DNA ANALYSIS ON FINE-NEEDLE ASPIRATES FROM BREAST TUMORS
M.Us-Krasovec, A.Pogacnik, R.Golouh, B.Skrbinc, M.Bracko
Institute of Oncology, Ljubljana, Slovenia

63. FLOW CYTOMETRIC ANALYSIS OF ACTIVATED SLUDGE WITH rRNA- TARGETED OLIGONUCLEOTIDE PROBES
G.Wallner, R.Amann
GSF-Forschungszentrum für Umwelt und Gesundheit, AG Durchfluázytometrie, and TU München, Lehrstuhl für Mikrobiologie

64. FLOW CYTOMETRIC MEASUREMENT OF HYDROGEN PEROXIDE (H2O2) BURST IN CEREBROSPINAL FLUID (CSF) CELLS
U.Zettl1, E.Mix2, G.Rothe3, H.-P.Hartung1
1 Dept. of Neurology, Universty of Würzburg, 2 Dept. of Neurology, Hosp. Nerv. Dis., University of Rostock, 3 Dept. of Clinical Chemistry, University of Regensburg, Germany

DGfZ Abstracts