Deutsche Gesellschaft für Zytometrie (DGfZ)

DGfZ Abstracts

Full Text

The abstract are printed in the: Proceedings of the DGZ Heidelberg Meetings, DKFZ, Heidelberg 1996, ISSN 0949 - 5347

=== ORAL PRESENTATIONS ===

=== Tumor Biology and Tumor Diagnostics ===

2. IMPROVED SINGLE LASER MEASUREMENT OF TWO CELLULAR ANTIGENS AND DNA-PLOIDY BY THE COMBINED USE OF PROPIDIUM IODIDE AND TO-PRO-3 IODIDE
Willem E. Corver, Gert Jan Fleuren and Cees J. Cornelisse
Department of Pathology, Faculty of Medicine, Leiden University, Leiden, the Netherlands

3. GRADE OF ANEUPLOIDY (5c- AND 9c-EXCEEDING RATE) AS A PROGNOSTIC MARKER FOR BREAST CANCER
Coumbos, A., Ruhnke, M., Yildirim, S. and Kühn, W.
Dept. of Obstetrics and Gynecology, Klinikum Benjamin Franklin, Free University of Berlin,Hindenburgdamm 30, 12200 Berlin, Germany

.4 THE VALUE OF FLOW CYTOMETRY IN STUDYING MALIGNANT GLIOMAS
V. Ehemann, B. Eifert*, K. Munkel*, A. Lange, H. F. Otto
Institute of Pathology, and Department of Neurosurgery*, University of Heidelberg, Germany

5. STANDARDIZATION OF FLOW-CYTOMETRIC DUAL PARAMETER-DNA MEASUREMENTS ON HUMAN COLON CARCINOMA
Ruth Knüchel, H.Zirngibl*, F.Hofstädter
Institute of Pathology and Dept. of Surgery, University of Regensburg, Germany

6. METRIC ANALYSIS OF MITOCHONDRIAL FUNCTION OF ONCOGENE-TRANSFORMED FIBROBLASTS ISOLATED FROM 2D- AND 3D-CULTURE
L.A. Kunz-Schughart1,2, R.C. Habbersett1, and J.P. Freyer1
1Los Alamos Natl. Lab., Life Sciences Division, Los Alamos, NM 87545, USA and 2Institute of Pathology, University of Regensburg, 93042 Regensburg, Germany

7. CHARACTERISATION OF APOPTOSIS IN LUTEAL CEllS FROM EARLY OVARIAN CYCLE STAGES BY FLOW CYTOMETRY
Bertold Löhrke, Ralf Pöhland, Torsten Viergutz Research Institute of Animal Biology, Dummerstorf-Rostock

8. A NOVEL, MEMBRANE RECEPTOR BASED RETROVIRAL VECTOR FOR FANCONI ANEMIA GROUP C GENE THERAPY
Andreas W. Machl, Simone Planitzer, Manfred Kubbies
Boehringer Mannheim GmbH, Biotechnology Therapeutics Research Center, 82377 Penzberg, Germany

9. ANEUPLOIDY OF HUMAN GRANULOSA CELLS IN FOLLICULAR FLUIDS OF IN-VITRO FERTILIZATION (IVF) PATIENTS
P. Melsheimer1, K. Grunwald2, K. Feldmann2, K. Klinga2, T. Rabe2, B. Runnebaum2, H.H. Rummel1
1 Abteilung fur Gynäkologische Morphologie, Universitäts-Frauenklinik, Vossstr. 9, D69115 Heidelberg, 2 Abteilung fur Gynäkologische Endokrinologie, Universitäts-Frauenklinik Heidelberg

10. THE INCIDENCE OF ANEUPLOIDIES IN MULTIPLE MYELOMA DOES NOT CORRELATE WITH STAGE OF DISEASE
R. Nowak, U. Oelschlägel, U. Range and G. Ehninger
Medical Clinic I, Technical University Dresden, 01307 Dresden

11. NON-SYNGENEIC CELL MODELS FOR SCREENING OF DNA-REPAIR RELEVANT GENES IN FANCONI'S ANEMIA VIA DIFFERENTIAL DISPLAY TECHNIQUE
Simone Planitzer, Andreas W. Machl and Manfred Kubbies
Boehringer Mannheim GmbH, Biotechnology Therapeutics Research Center, 82377 Penzberg, Germany

12. ALTERATIONS IN CELLULAR MITOCHONDRIAL POTENTIAL UNDER INFLUENCE OF APOPTOSIS INCUCING DRUGS
Pogrebniak A, Stoetzer OJ, Wilmanns W, Nüssler V
GSF-Research Center for Health and Enviroment, Institute for Clinical Hematology, Munich

13. DNA DIPLOIDY - A RARE FEATURE OF OVARIAN CARCINOMA?
Reich O, Pürstner P.
Department of Obstetrics and Gynecology, University of Graz, Austria

14. DISCRIMINIATION OF OVARIAN TUMORS BY DNA CYTOMETRY
M.Ruhnke, A. Ditzenbach and W. Kühn
Department of Gynecologic Morphology, Academic Medical Center Benjamin Franklin. Free University of Berlin, Hindenburgdamm 30, D-12200 Berlin

15. ANGIOGENESE IN OVARIALKARZINOMEN: ERSTE ERFAHRUNGEN MIT BILDANALYTISCHER QUANTIFIZIERUNG DER ENDOTHELIALEN IMMUNOREAKTIVITAET UND IHRER MOEGLICHEN PROGNOSTISCHEN BEDEUTUNG
W. Schöll, O. Reich, D. Pieber, F. Gücer
Geburtshilflich-Gynäkologische Univ.-Klinik, Graz

16. NUMERICAL ABERRATIONS OF CHROMOSOME 17 IN DIPLOID AND ANEUPLOID TUMORS.
Schnekenbühl S. Polackova J, Nagel E, Hemmer J.
Divison of Tumor Biology, University of Ulm

17. PROLIFERATION KINETIC IN GASTRIC CANCER"IN VIVO"
A. Sendler* , K.-P. Gilbertz , A. Rhein , K. Matzen*, U. Fink*, l. Becker+, D. van Beuningen
*Chirurgische Klinik und Institut für Pathologie der TU München, Klinikum rechts der Isar, 81644 München, Institut für Radiobiologie, Akademie des Sanitäts- und Gesundheitswesen der Bundeswehr, 80901 München, Germany

=== Immunophenotyping and Analysis of Cell Populations ===

18. FLOW CYTOMETRIC ANALYSIS OF LYMPHOMA CELL IMMUNOPHENOTYPE USING KNOWLEDGE BASED CLUSTER SOFTWARE
S. Barlage, G. Rothe, G. Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany

19. FACS-ANALYSIS OF SELECTIVE SKIN INFILTRATING BLOOD CELLS DUE TO AN ANTIGENIC CHALLENGE IN VIVO
B&oouml;hm I., Bauer R.
Universitäts-Hautklinik Bonn, Sigmund-Freud-Str. 25, D-53105 Bonn

20. CD69 EXPRESSION AS A MEASURE FOR CHANGES IN PROLIFERATION OF LYMPHOCYTES IN HIV-AB+ PATIENTS AND PATIENTS WITH CFIDS
Janssen M., Braun P. and Knechten H.
PZB Aachen, Blondelstr. 9, 52062 Aachen, Germany

21. BLOCKING OF THE HIV-1 ENVELOPE GLYCOPROTEIN (GP120) BINDING TO CD4+ T LYMPHOCYTES BY ANTI-HIV MONOCLONAL ANTIBODIES
Kröpelin, M., Süsal, C., Daniel, V., and Opelz, G.
University of Heidelberg, Institute of Immunology, 69120 Heidelberg, Germany

22. CYTOMETRIC ANALYSIS OF ANTIBODY-SECRETING CELLS R. A. Manz1, A.Thiel1, S. Miltenyi2 and A. Radbruch1,
1Institute for Genetics, University of Cologne, Germany, 2Miltenyi Biotec GmbH, Bergisch Gladbach, Germany

23. An IMPROVED FLOW CYTOMETRIC WHOLE BLOOD ASSAY FOR RETICULATED PLATELETS
G.B. Matic, G. Rothe, G. Schmitz
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany

24. IN VITRO INVESTIGATIONS OF LYMPHOCYTE HOMING IN INFLAMMATORY RHEUMATIC DISEASES USING FLOW CYTOMETRY AND IMAGE ANALYSIS
J. Neumüller (1), J. Menzel (2), A. Dunky (3)
(1) Ludwig Boltzmann Institute for Rheumatology and Balneology, Vienna, (2) Institute of Immunology, University of Vienna, (3) 5th Department for Internal Medicine, Wilhelminenspital, Vienna, Austria

25. DETECTION OF CYTOKERATIN 8-18 AND P53 POSITIVE CELLS BY FLOW CYTOMETRY IN HUMAN RENAL CARCINOMA
Eugen Plas, Ruth Jilch*, Sonja Thury*, Michael Fischer*, Heinz Pflüger
Dept. of Urology and LBI for Urology and Andrology, Lainz Hospital *Dept. of Laboratory Medicine, Lainz Hospital Vienna, Austria

26. Institute for Genetics, University of Cologne, Germany, #Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
IFN-y AND IL-10 CAN BE DETECTED ON THE SURFACE OF SECRETING CELLS BY MAGNETOFLUORESCENT LIPOSOMES: SPECIFIC MARKERS FOR CYTOKINE SECRETION
A. Scheffold&, M. Assenmacher&. J. Schmitz#, S. Miltenyi# and A. Radbruch&

27.IMMUNOLOGICAL ALTERATIONS DURING PEDIATRIC CARDIAC AND VASCULAR SURGERY: THE INFLUENCE OF THE CARDIO-PULMONARY BYPASS (CPB)
Tarnok A., Hambsch J., Kinzel P., Borte P., Sack U., Schneider P.
Pediatric Cardiology, Heart Center Leipzig - University Hospital - Russenstr. 19, 04275 Leipzig, Germany

28. MEASUREMENT OF PROTEIN-PROTEIN-INTERACTION BY FLOW CYTOMETRY USING FLUORESCENT MICROBEADS
Tarnok A.1, Nöhrenberg U.2, Schuhmacher S.2, Vollmer H.J.2, Rathjen F.2
Pediatric Cardiology, Heart-Center Leipzig - University Hospital - Russenstr. 19, Leipzig 2 Max-Delbruck-Center for Molecular Medicine, Berlin-Buch, Germany

29. FLOW CYTOMETRIC CHARACTERIZATION OF HUMAN TROPHOBLASTS AFTER ISOLATION FROM FRESH PLACENTAL TISSUE
Thieme B., Koop F., Harbeck N., Kolben M., *Ugele B., Schneider KTM., Graeff H. and Schmitt M.
Departments of Obstetrics & Gynecology, Technical University, * Ludwig- Maximilians-University, Munich, Germany

30. CHARACTERIZATION OF A PATIENT WITH REDUCED PLATELET CD36 EXPRESSION AND ENHANCED GLYCOPROTEIN Ib-lX EXPRESSION.
A. Zwick, E. Orso, J. Stöhr, E. Ajzner, S. Barlage, W. Drobnik, G. Rothe, C. Aslanidis and G. Schmitz.
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg,D-93042 Regensburg, Germany

=== Image Analysis and Cell Topology ===

31. Microscope control, image acquisition and visualization in a network environment: towards "online", long distance telemicroscopy
by Matthias Nagorni, Joachim Bradl, Bernhard Schneider, Michael Hausmann, Christoph Cremer
Institute of Applied Physics, University of Heidelberg, Albert-Ueberle-Str. 3-5, D-69120 Heidelberg

32. Axialtomographical Microscopy for High Resolution Studies of the Interphase Genome Organization
B.Rinke1, P.Edelmann1, J.Bradl1, A.Esa1, B.Schneider1, M.Hausmann1, C.Cremer1,2
Institute of Applied Physics, University of Heidelberg 2 Interdisciplinary Centre for Scientific Computing (IWR), University of Heidelberg

33. A NEW PRINCIPLE IN CONFOCAL MICROSCOPY: INVARIABLE LIGHTPATH LASER-SCANNING MICROSCOPY
VON STELDERN DU,1, Milicev M., 2
1Biotechnologie CONSULTING, D-69 1 8 1 LEIMEN 2 MG MICROSCOPE GMBH, D-69245 BAMMENTAL

34. CONFOCAL 3D-MICROSCOPY: ACQUISITION, DOCUMENTATION AND PRESENTATION
P.J.,S. Hutzler,
GSF-Forschungszentrum, Oberschleißheim

35. MORPHOMETRIC ANALYSIS OF AgNOR PROTEINS FOR CELL DOUBLING TIME EVALUATION IN TUMOR PATHOLOGY
Massimo Derenzini
Dipartimento di Patologia Sperimentale, Universita di Bologna Italy

=== Standardization and Quality Control ===

36. QUALITY ASSESSMENT FOR IMMUNOPHENOTYPING OF LEUKEMIAS AND LYMPHOMAS
J.W. Gratama1 and J.C. Kluin-Nelemans 2, for the Dutch Cooperative Study Group on Immunophenotyping of Hematological Malignancies (SIHON)
1 Department of Clinical and Tumor Immunology, Daniel den Hoed Cancer Center, Rotterdam, and Department of Hematology, University Hospital, Leiden, the Netherlands

37. EXPERIENCES FROM COLLABORATIVE STUDIES ON QUALITY ASSURANCE OF FLOW CYTOMETRIC DNA MEASUREMENTS
Friedrich J. Otto, Ulrike Westhoff
Fachklinik Hornheide, Abteilung fur Tumorforschung, D-48157 Münster

38. EUROPEAN CONSENSUS PROTOCOL FOR THE FLOW CYTOMETRIC CHARACTERIZATION OF PLATELET FUNCTION
G. Rothe for the European Working Group on Clinical Cell Analysis - Task Force on Platelet Analysis (J. Kappelmayer, R. Lenkei, G. Rothe, G. Schmitz)

39. USE OF ELECTRONIC CELL COUNTER ANALYSER SYSTEMS FOR QUALITY CONTROL
5W-Rule: Who did what, when, with what and why? (GLP)
Schärfe System GmbH, Emil-Adolff-Straße 14, D-72760 Reutlingen

40. EXTERNAL QUALITY ASSESSMENT SCHEME "RETICULOCYTES" (INSTAND E.V.)- A THREE YEARS EXPERIENCE
Stefan Serke,
Virchow-Klinikum der Humboldt-Universität zu Berlin, Abteilung Innere Medizin und Poliklinik, Hämatologie und Onkologie, Augustenburger Platz 1, 13353 Berlin, FAX: +49.30.4505.3914

=== Microorganisms and Biotechnology ===

41. FLOW CYTOMETRY WITH PHENOL DEGRADING BACTERIA AFTER IN SITU HYBRIDIZATION AND DAPI STAINING
C. HERRMANN
Abteilung Biotechnologie, Fakultät für Biowissenschaften, Pharmazie und Psychologie, Universitaml;t Leipzig, Permoserstr. 15, 04318 Leipzig

42. LIGHT SCATTER MEASUREMENTS OF BACTERIA: MODEL CALCULATIONS AND EXPERIMENTS
Andreas Lösche
Projektgruppe Biosignale, Abteilung Biotechnologie, Fakultät für Biowissenschaften, Pharmazie und Psychologie, Universität Leipzig, Permoserstr. 15, D-043 18 Leipzig

43. MICROORGANISMS IN CONCENTRATED FRUIT JUICES
Moustafa M., Wartenberg R. and Sachsse W.
Depart. of cytogenetic Univ. of Mainz

44. ASSESSMENT OF BACTERIAL VIABILITY STATUS BY FLOW CYTOMETRY AND SINGLE CELL SORTING
G. Nebe-von Caron*, P. Stephens and R.A.Badley
Unilever Research, Colworth Laboratory, Sharnbrook, Beds., MK44 1LQ, UK

45. GERMINATION AND OUTGROWTH OF SPORES CHARACTERIZED BY FLUORESCENT PROBES AND FLOW CYTOMETRY
G. Nebe-von Caron* and W.A. Anderson,
Unilever Research, Colworth Laboratory. Sharnbrook. Beds., MK44 1LQ United Kingdom

46. FLOW CYTOMETRY OF MESOPHYLL AND BUNDLE SHEATH CHLOROPLASTS OF VARIOUS PLANTS WITH C4 PHOTOSYNTHESIS
E. Pfündel 1,2 and A. Meister1,
1 Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstr. 3, D- 06466 Gatersleben. 2 Universität Leipzig, Institut fur Botanik, Johannisallee 21, D-04103 Leipzig

47. THREE-LASER FLOW CYTOMETRY FOR SIMULTANEOUS MEASUREMENT OF PHOTOSYNTHESIS PIGMENTS AND PROTEIN CONTENT OF PHYTOPLANKTON POPULATIONS IN LAKES AND RIVERS
Schäfer,H.1,2; Bruckmeyer,B.1,2;Steinberg, C.2 and Beisker, W.1
(1): GSF-Durchfluszlig;zytometrie Ingolstädter Landstr 1, D-85764 Neuherberg, Germany (2): Institut für Gewässerökologie und Binnenfischerei Müggelseedamm 310, D-12587Berlin, Germany

48. NON-INVASIVE BIOPROCESS MONITORING: POPULATION DYNAMICS
Bernhard Sonnleitner
Institut für Biotechnologie, ETH Zürich

49. OUTGROWTH AND VITALITY DISTRIBUTIONS OF INJURED LACTOBACILLUS PLANTARUM CELLS ASSESSED BY FLOW CYTOMETRY
Ueckert J.and Ter Steeg,P.F.
Unilever Research Laboratorium P.O. Box 114, 3130 AT Vlaardingen, NL

=== New Technologies, Procedures and Concepts ===

50. FLUORESCENCE LIFE-TIME MEASUREMENTS IN FLOW CYTOMETRY PRINCIPLES AND FIRST APPLICATIONS
Beisker, K. and Klocke, A.
GSF-Durchflußzytometrie Ingolstädter Landstr 1, D-85764 Neuherberg, Germany

51. COMPREHENSIVE MONITORING OF CELLS IN CULTURE: THE PHYSIOCONTROL-MICROSYSTEM
M. Brischwein a*, A. Schwinde a, W. Baumann a, R. Ehret a, P. Seidl b and B. Wolf a
a AG Medizinische Physik und Elektronenmikroskopie, Institut für Immunbiologie, Universität Freiburg, Stefan-Meier-Str. 8, 79104 Freiburg; Germany,
b Physikalisch Technische Studien GmbH, Leinenweberstr. 16,, 79108 Freiburg, Germany
Keywords: cellular biosensor, Physiocontrol-Microsystem, transducer array

52. MEASUREMENT OF INTRACELLUAR ENZYME ACTIVITY BY THE AID OF MULTIPARAMETRIC FLOW CYTOMETRY
Rolf Eckhardt,
Coulter Electronics GmbH, Europark Fichtenhain B13, 47807 Krefeld

53. FORMATION OF CELLULAR AGGREGATES DURING ROUTINE IMMUNOSTAINING: RELEVANCE OF THE FCY RECEPTOR IIA POLYMORPHISM FOR CLINICAL FLOW CYTOMETRY
J.W. Gratama,1 R. v.d. Linden,l C. de Beukelaer,1 J.G.J. van de Winkel 2 and R.L.H. Bolhuis1
Department of Clinical and Tumor Immunology, Daniel den Hoed Cancer Center, Rotterdam and 2 Department of Immunology, University Hospital, Utrecht, the Netherlands

54. LABELING AND ANALYSIS OF RECOMBINANT HIRUDUIN USING FLOW CYTOMETRY
Malsch, L. Piazolo, W. Lianchun, A. Timmermann and J. Harenberg
I. Medizinische Klinik, Fakultät für klinische Medizin Mannheim der Universität Heidelberg, Theodor Kutzer Ufer, 68167 Mannheim

55. ANALYSIS OF THE ROLE OF THE FAC-GENE IN THE REGULATION OF THE CELL CYCLE AND APOPTOSIS WITH A NOVEL FLOW CYTOMETRIC METHOD
Martin Poot, Frank Kruyt2, Hans Joenje2, Victoria L. Singer and Richard P. Haugland.
Molecular Probes, Inc., Eugene, OR 97402, U.S.A. und Department of Human Genetics 2, Free University of Amsterdam,1081 BT Amsterdam, The Netherlands

56. DISSECTION OF APOPTOTIC PATHWAYS IN LIVE CELLS BY MITOTRACKER RED CM-XROS AND SYTO DYE FLOW CYTOMETRY
Martin Poot, Lisa L. Gibson, Victoria L. Singer and Richard P. Haugland.
Molecular Probes, Inc., Department of Biosciences, Eugene, OR 97402, U.S.A.

57. EXCIMER FLUORESCENCE COMPARED TO DEPOLARIZATION IN THE FLOW CYTOMETRIC CHARACTERIZATION OF LATERAL MEMBRANE MOBILITY
G. Rothe, B. Schäfer, G. Schmitz
Institute for Clinical Chemistrv. University of Regensburg, Germany

58. FLOW CYTOMETRY OF ALKALINE AND ACID PHOSPHATASE ACTIVITIES - COMPARISON OF THE DIAZOIC COUPLING WITH THE METHYLUMBELLIFERON METHOD
E. Severin und E. Seidler,
Institut fur Strahlenbiologie der Universität Münster, Robert-Kochstr. 43, D-48149 Münster

59. PHASE-SENSITIVE FLOW CYTOMETRY: LIFETIME-BASED TECHNOLOGY FOR ANALYZING CELLS\CHROMOSOMES LABELED WITH FLUORESCENT PROBES
John A. Steinkamp
Life Sciences Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA

60. PROGNOSTIC SURVIVAL ESTIMATION OF COLORECTAL CANCER PATIENTS BY CLASSIF1 ANALYSIS OF MULTIPARAMETER DATA FROM G6PDH OXYGEN INSENSITIVITY TEST, SOD AND LIPID PEROXIDATION DETERMINATION ON HISTOPHATOLOGICAL CRYOSECTIONS.
G.Valet1), B.E.M. Van Driel2), H.Lyon3), U.Hansen3), J.Song2), C.J.F. Van Noorden2)
1) Max-Planck-lnst.Biochemie, 82152 Martinsried, Germany, 2) Lab.Cell Biol.and Histol, Academic Med.Center, Univ.Amsterdam, NL, 3) Dep.Pathol., Kobenhavns Kommunes Hvidovre Hosp., Univ.Copenhagen, Denmark

61. FLOW CYTOMETRIC ASSESSMENT OF SHOCK WAVE INDUCED ALTERATIONS OF PLASMA MEMBRANE POTENTIAL.
G. Woelfl, J. Bette, E. Endl, F. Hofstädter,
Institute of Pathology, Univeristy of Regensburg, Germany

=== POSTERS ===

62. HIGH SPEED FISH FOR REPETITIVE AND PAINTING PROBES
M. DURM1,3, D. WOLF1, K. ALDINGER1, F.-M.HAAR1,M. HAUSMANN1, C. CREMER1,2

63. FAST FISH DETECTION AND AUTOMATED DIGITAL IMAGE ANALYSIS OF NUMERMICAL CHROMOSOMAL ABERRATIONS IN BONE MARROW AND PERIPHERAL BLOOD INTERPHASE NUCLEI OF HEMATOONCOLOGICAL PATIENTS
A. Esa1. L. Trakhtenbrot2. M. Hausmann1, J. Ben-Bassat2, C. Cremer1
1 = Institute of Applied Physics, University of Heidelberg, Albert-Ueberle-Str. 3- 5, D-69120 Heidelberg, F.R. Germany 2 = The Institute of Hematology, The Chaim Sheba Medical Center, Tel Hashomer, Israel

64. AUTOMATIC OBSERVATION OF THE DROPLET BREAKOFF POINT
Joachim W. Ellwart. Ingolf Karls*
GSF-National Research Center for Environment and Health, Institute of Experimental Hematology, Marchioninistr. 25, D-81377 Munich, Germany *Siemens AG, Munich, Germany

65. CELL CULTURE TESTING OF BONE REPLACEMENT MATERIALS GLASS IONOMER CEMENT AND POLYMETHACRYLATE
Dr.Dr. S. Hassfeld, Dr. R. Frombach, Dr.Dr. R. Wiedenmann, PD Dr.Dr. J. Zöller
Oral and Maxillofacial Surgery, Ruprecht-Karls-University Heidelberg Im Neuenheimer Feld 400, 69120 Heidelberg, Germany

66. CHANGES OF LYMPHOCYTE SUBSETS AND THEIR CELL HOMING RECEPTOR CAPACITY IN HUMAN BLOOD AFTER TOTAL SLEEP DEPRIVATION
T.O. Kleine*, W. Schreiber**, J. Albrecht***
*Funktionsbereich Neurochemie,**Klinik fur Psychiatrie, Med. Zentrum fur Nervenheilkunde, Philipps-Universitat Marburg; ***Becton Dickinson, Heidelberg

67. TRANSFER OF LYMPHOCYTES THROUGH THE BLOOD/CSF BARRIER OF HUMANS
T.O. Kleine*, H.J. Werner*, J. Albrecht**
*Med. Zentrum fur Nervenheilkunde, Funktionsbereiech Neurochemie, Philipps-Universitat Marburg ** Becton Dickinson, Heidelberg

68. DETECTION OF H202/L-HISTIDINE INDUCED CHROMOSOME ABERRATIONS BY MEANS OF SLIT-SCAN FLOW FLUOROMETRY
Heiko Lentfer, Dietmar Wolf, Martin Crone, Klaus Aldinger, Michael Hausmann, Christoph Cremer
Institute of Applied Physics, University of Heidelberg, Albert-Ueberle-Str. 3-5, D-69120 Heidelberg, F.R. Germany

69. A NEW FLOW CYTOMETRIC ASSAY FOR BASOPHIL DEGRANULATION
Thomas Nebe, Karin Hartmann and Waltraut Pfirrmann
Institut für Klinische Chemie. Klinikum Mannheim, D-68135 Mannheim

70. COMPARISON OF DIFFERENT METHODS, TARGETS AND INDUCERS OF APOPTOSIS
Thomas Nebe1, Jutta Wucher1, Monika Bühl2, Gerolf Maier1, Ingrid Brechtel1 and Werner Hirt2
1 Institut fur Klinische Chemie, Klinikum Mannheim, D-68135 Mannheim and 2 ORPEGEN Pharma, D-69115 Heidelberg, Germany

71. COMPARISON OF DIFFERENT TECHNIQUES TO ANALYSE MULTI-DRUG RESISTANCE
Thomas Nebe1, Susan Kranzpiller1, Verena Zunftmeister1 M. Cianfriglia3, and Alexandra Dorn-Beineke2
1 Institut für Klinische Chemie, Klinikum Mannheim, D-68135 Mannheim, 2 Labor Prof. Seelig, Kriegstral3e 99, D-76133 Karlsruhe, Germany and 3 Inst. f. Sanita Superiore di Roma, Italy

72. PROLIFERATION VERSUS APOPTOSIS: THE BIOLOGICAL ACTIVITY OF THYMIC PEPTIDES ON LYMPHOCYTES OF HEALTHY DONORS AND HIV PATIENTS
Thomas Nebe1, Gerolf Maier, Karin Hartmann, Ingrid Brechtel and Gerhard Becker2
1 Institut fur Klinische Chemie, Klinikum Mannheim, D-68135 Mannheim and 2 Sanorell Pharma,D-72270 Baiersbronn, Germany

73. PROLIFERATION, APOPTOSIS OR PHAGOCYTOSIS: THE SUPPRESSIVE EFFECT OF AUTOANTIBODIES ON LYMPHOCYTES OF HEALTHY DONORS AND HIV PATIENTS
T.Nebe1, G.Maier1, M.Müller1, I.Brechtel1, K.Hartmann1, F.Thienel2, B.Buchholz3, K.Friese4, A.Willer5 and K.P.Becker6
1Inst für Klinische Chemie, 2I.Med.Klinik, 3Kinderklinik, 4Frauenklinik 5III.Med. Klinik, 6Institut fur Mikrobiologie und Hygiene, Klinikum Mannheim, D-68135 Mannheim, Germany

75. INDUSTRIAL PROCESS ANALYSIS: FLOW CYTOMETRY IN THE BREWING TECHNOLOGY
Petzold, Louise1; Müller, Susann1, Hutter, Karl-Josef2, Bley, Thomas 3
1 Universität Leipzig, Abteilung Biotechnologie, Permoserstr.15, Leipzig 2 DKFZ Heidelberg, Im Neuenheimer Feld 280, Heidelberg 3 TU Dresden. Institut Lebensmittel- und Bioverfahrenstechnik, Dresden

76. ANALYSIS OF CHROMOSOMAL IMBALANCES IN BIOPSIES FROM ORAL SQUAMOUS CELL CARCINOMAS AND PRECURSOR LESIONS BY A COMBINATION OF MICRODISSECTION, UNIVERSAL DNA- AMPLIFICATION AND COMPARATIVE GENOMIC HYBRIDIZATION (CGH)
Martin Scheer1,2,4; Ruthild Weber 2,4; Christof Hofele 1; Stefan Joos 4; 1. Antonio Born 3; Peter Lichter 4; Joachim Zöller 1; Thomas Cremer 2,5
1 Department of Oral and Maxillofacial Surgery, 2 Institute of Human Genetics and 3 Institute of Pathology, University of Heidelberg, 4 German Cancer Research Center, 69120 Heidelberg, Germany and 5 Institute of Anthropology and Human Genetics, 80333 München. Germany

77. DISTANCE MEASUREMENTS OF SINGLE TARGETS IN INTERPHASE NUCLEI BY WAVEFIELD MICROSCOPY
Bernhard Schneider 1, Joachim Bradl 1, Ingolf Kirsten 1, Matthias Nagorni 1, Bernd Rinke 1, Michael Hausmann 1, Christoph Cremer1,2
1, Institut für Angewandte Physik, Universität Heidelberg, Albert-Ueberle-Str. 3- 5, D-69120 Heidelberg 2 Interdisziplinäres Zentrum far wissenschaftliches Rechnen (IWR), Universität Heidelberg.
key words:
distance measurements in fluorescence microscopy, axialtomography

78. HISTKOM - FIELDTEST IN GERMANY FOR TELEPATHOLOGY AND TELECYTOLOGY
P. Schwarzmann, B. Binder, J. Burkart, J. Schmid
Institut fur Physikalische Elektronik, Universität Stuttgart

DGfZ

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