This is a table of characteristics for fluorochromes used in flow cytometry, within
groups, roughly in order of excitation wavelength.
Colors you might see with a capable
browser are only a rough approximation.
Probe |
Ex (nm) |
Em (nm) |
MW |
Notes |
Reactive and conjugated probes |
Hydroxycoumarin |
325 |
386 |
331 |
Succinimidyl ester |
Aminocoumarin |
350 |
445 |
330 |
Succinimidyl ester |
Methoxycoumarin |
360 |
410 |
317 |
Succinimidyl ester |
Cascade Blue |
375;400 |
423 |
596 |
Hydrazide |
Lucifer yellow |
425 |
528 |
|
|
NBD |
466 |
539 |
294 |
NBD-X |
R-Phycoerythrin (PE) |
480;565 |
578 |
240,000 |
|
PE-Cy5 conjugates |
480;565;650 |
670 |
|
aka Cychrome, R670, Tri-Color, Quantum Red |
PE-Cy7 conjugates |
480;565;743 |
767 |
|
|
Red 613 |
480;565 |
613 |
|
PE-Texas Red |
Fluorescein |
495 |
519 |
389 |
FITC; pH sensitive |
BODIPY-FL |
503 |
512 |
|
|
Cy3 |
512;552 |
565,615 |
|
|
TRITC |
547 |
572 |
444 |
TRITC |
X-Rhodamine |
570 |
576 |
548 |
XRITC |
Lissamine Rhodamine B |
570 |
590 |
|
|
PerCP |
490 |
675 |
|
Peridinin chlorphyll protein |
Texas Red |
589 |
615 |
625 |
Sulfonyl chloride |
Cy5 |
625-650 |
670 |
|
|
Cy7 |
743 |
767 |
1,001 |
|
Allophycocyanin (APC) |
650 |
660 |
104,000 |
|
TruRed |
490,675 |
695 |
|
PerCP-Cy5.5 conjugate |
APC-Cy7 conjugates |
650;755 |
767 |
|
PharRed |
Nucleic acid probes |
Hoechst 33342 |
343 |
483 |
616 |
AT-selective |
DAPI |
345 |
455 |
|
AT-selective |
Hoechst 33258 |
345 |
478 |
624 |
AT-selective |
SYTOX Blue |
431 |
480 |
~400 |
DNA |
Chromomycin A3 |
445 |
575 |
|
CG-selective |
Mithramycin |
445 |
575 |
|
|
YOYO-1 |
491 |
509 |
1270.65 |
|
SYTOX Green |
504 |
523 |
~600 |
DNA |
SYTOX Orange |
547 |
570 |
~500 |
DNA |
Ethidium Bromide |
493 |
620 |
394.31 |
|
7-AAD |
546 |
647 |
|
7-aminoactinomycin D, CG-selective |
Acridine Orange |
503 |
530/640 |
|
DNA/RNA |
TOTO-1, TO-PRO-1 |
509 |
533 |
|
Vital stain, TOTO: Cyanine Dimer TO-PRO: Cyanine Monomer |
Thiazole Orange |
510 |
530 |
|
|
Propidium Iodide (PI) |
536 |
617 |
668.4 |
|
TOTO-3, TO-PRO-3 |
642 |
661 |
|
|
LDS 751 |
543;590 |
712;607 |
471.98 |
DNA (543ex/712em), RNA (590ex/607em) |
Cell function probes |
Indo-1 |
361/330 |
490/405 |
1010 |
AM ester. Low/High Ca++, |
Fluo-3 |
506 |
526 |
855 |
AM ester. pH > 6 |
DCFH |
505 |
535 |
529 |
2'7'Dichorodihydrofluorescein, oxidized form |
DHR |
505 |
534 |
346 |
Dihydrorhodamine 123, oxidized form, light catalyzes oxidation |
SNARF |
548/579 |
587/635 |
|
pH 6/9 |
Fluorescent Proteins |
Y66F |
360 |
508 |
|
|
Y66H |
360 |
442 |
|
|
EBFP |
380 |
440 |
|
(Clonetech) Quantum yield 0.18 |
Wild Type |
396,475 |
508,503 |
|
|
GFPuv |
385 |
508 |
|
(Clonetech) |
ECFP |
434 |
477 |
|
(Clonetech) Quantum yield 0.40 |
Y66W |
436 |
485 |
|
|
S65A |
471 |
504 |
|
|
S65C |
479 |
507 |
|
|
S65L |
484 |
510 |
|
|
S65T |
488 |
511 |
|
|
EGFP |
489 |
508 |
|
(Clonetech) Quantum yield 0.60 |
EYFP |
514 |
527 |
|
(Clonetech) Quantum yield 0.61 |
DsRed |
558 |
583 |
|
(Clonetech) Quantum yield 0.29 |
Other probes |
Monochlorobimane |
380 |
461 |
226 |
Glutathione probe |
Calcein |
496 |
517 |
623 |
pH > 5 |
Legend:
- Ex: Peak excitation wavelength (nm)
- Em: Peak emission wavelength (nm)
- MW: Molecular weight
|