1% PARAFORMALDEHYDE FIXATIVE FOR SURFACE IMMUNOPHENOTYPING
Contributors: University of Florida School of Dental Medicine Flow Cytometry Facility
E-mail: SDMFACS@path.dental.upenn.edu
URL: http://biochem.dental.upenn.edu/~sdmfacs/
Procedure:
  1. IN A FUME HOOD, heat 200 mL of deionized water to near boiling. DO NOTBOIL.
  2. Add the following while stirring:

    2.68 g sodium cacodylate Sigma C 0250
    1.9 g sodium chloride
    2.95 g paraformaldehyde Fisher T353-500


  3. Cool to room temperature, raise volume to 250 mL, and adjust pH to 7.2 to 7.4.
  4. Store at 4C in a foil wrapped bottle for no more than 1 month.
From Babcock, G. F., and Dawes, S. M. (1994). Methods in Cell Biology 41 (Flow Cytometry Supplement) 84.

MAM 5/19/97