4% Paraformaldehyde Fixative for Cytoplasmic Staining, Including Tunel
Contributors: University of Florida School of Dental Medicine Flow Cytometry Facility
E-mail: SDMFACS@path.dental.upenn.edu
URL: http://biochem.dental.upenn.edu/~sdmfacs/

MAKES 250 mL

 
  1. IN A FUME HOOD, heat 200 mL of deionized water to near boiling. DO NOT BOIL.
  2. Add the following while stirring:
        3.  
        2.68 g sodium cacodylate Sigma C 0250
        1.9 g sodium chloride
        11.8 g paraformaldehyde Fisher T353-500
         
       
  3. Cool to room temperature, raise volume to 250 mL, and adjust pH to 7.2 to 7.4.
  4. Store at 4C in a foil wrapped bottle for no more than 1 month.
Based on Babcock, G. F., and Dawes, S. M. (1994). Methods in Cell Biology 41 (Flow Cytometry Supplement) 84.

MAM 8/13/97