Application:
Monoclonal antibodies to p53, clone BP53.12, are used in immunohistochemistry to detect alterations in the p53 protein. P53 binds to a DNA consensus sequence, the p53 response element, and it regulates normal cell growth cycle events by activating transcription of genes, involved either in progression through the cycle, or causing arrest in the G1 phase when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the minute concentrations in normal cells, principally due to the increased half-life (4h) compared to that of the wild-type (20 min). P53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis
The BP53-12 monoclonal reacts with a C-terminal epitope of the 53kD gene product and this epitope is not destroyed by formalin-fixation and routine paraffin embedding. Microwave treatment is recommended for optimal staining.

Specifications:
Antibodies to p53, clone BP53-12, produces mouse IgG2a immunoglobulins directed against both wild and mutant forms of p53 protein. It is reactive in paraffin sections and Western blotting but not frozen sections. This monoclonal antibody may also be used in ELISA techniques. Typical working dilution in immunohistochemistry is 1:40

frozen sections	paraffin sections	flow cytometry	ELISA	Western Blotting
-	+	+	+	+

Immunohistochemical staining for p53 protein using BP53-12 on (b) a Reed-Sternberg cell in Hodgkin's Lymphoma (dia # 1)

References:
1. Vogelstein and Kinzler, 1992. Cell, 70: 523-526
2. Hollstein et al. (1991) Science, 253: 49-53
3. Lane, D.P., 1992. Nature, 358: 15-16.
4. Donehower et al., 1993. Biochemic. Biophys. Acta. 1155: 181-182

For research only. Not for use in humans. For in vitro use only.