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Product Information E2-E3
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Antigen distribution:
Insulin is a protein consisting of an a-chain of 21 amino acids and a b-chain of 30 amino acids which is produced in the beta cells of the islets of Langerhans in the pancreas.
Application:
Monoclonal antibody E2-E3, detects human insulin as has been demonstrated by ELISA and immunohistochemistry on formaldehyde-fixed, paraffin-embedded sections of human pancreatic tissue. In addition swine and bovine insulun can be detected.
Clone E2-E3 may be applied in the identification of insulinomas.
Specifications:
Clone E2-E3 produces mouse IgG1 immunoglobulins directed against the insulin molecule.
Typical working dilution in immunohistochemistry is 1:40
| frozen sections |
paraffin sections |
flow cytometry |
ELISA |
Western Blotting |
| + |
+ |
ND |
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ND |
Immunohistochemical staining for insulin in pancreas using clone E2-E3. (dia # 6).
References:
For research only. Not for use in humans. For in vitro use only.
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