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Product Information CD57
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Antigen distribution:
The CD57 myeloid associated glycoprotein, also known as HNK-1, is found on a subset of mononuclear cells with natural killer activity and on neuroectodermal cells expressing myelin associated glycoprotein.
Cells which co-express CD57 and CD8 antigens are a subset of suppressor/cytotoxic T cells. CD57 cells may play a role in graft rejection in acute graft versus host disease.
Application:
Clone NK-1 monoclonal antibodies are used in the analysis of NK cell-mediated cytotoxicity, in studies of NK cell and T cell subsets or for neural tissue glycoproteins.
NK-1 reacts with formalin and B5 fixed paraffin embedded sections to human NK cells and T-cell subsets similar to HNK-1 and Leu-7. It recognizes NK cells (large granular lymphocytes) in germinal centers, neural tissue glycoproteins and prostate antigens.
Specifications:
Clone NK-1 produces mouse IgM immunoglobulins directed against a 110 kD lymphocyte surface antigen. It is reactive in frozen and paraffin sections and flow cytometry.
Typical working dilution in immunohistochemistry is 1:40
| frozen sections |
paraffin sections |
flow cytometry |
ELISA |
Western Blotting |
| + |
+ |
+ |
ND |
ND |
Immunohistochemical staining showing CD57 (Leu-7)
positive rosettes using clone NK-1 in
Hodgkin's Lymphoma. (dia #9)
References:
1 Abo,T., et al. 1981, J. Immunology. 127. 1024
2 McGarry, RC., et al., 1983. Nature. 306. 376.
3 Lanier, LL.., et al. 1983. J. Immunolgy. 131. 1789
For research only. Not for use in humans. For in vitro use only.
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