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Product Information CD138
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Antigen distribution:
Monoclonal antibody B-B4 has been clustered as CD138, and recognizes the syndecan-1 proteoglycan expressed on human plasma cells, endothelial cells and fibroblasts. B-B4 is frequently used for detection of malignant plasma cells (1-5). It does not react with circulating B cells, T cells, monocytes, granulocytes and normal bone marrow cells. In addition, B-B4 is reactive with cytoplasmic lg+ and surface lg- plasma cells, indicating a specific reactivity with secreting plasma cells (6).
Application:
CD138 antibodies, clone B-B4, can be applied in immunohistochemistry using paraffin embedded tissue sections. Immunohistochemical application of antibody B-B4 includes reactivity with B cells in lymphoid follicles, plasma cells, epithelium/endothelium in various organs. CD138 antibodies are used in the detection and monitoring of multiple myeloma, a lympho-proliferative B cell disease, in which malignant plasma cells produce large amounts of immunoglobulins
Specifications:
Clone B-B4 IgG1 immunoglobulins have been officially assigned to CD138 during the 6th Workshop on Human Leucocyte Antigens,1996 (6). Syndecan-1 is a 30.5 kDa heparan sulphate proteoglycan, probably involved in cellular matrix formation. It is reactive in paraffin sections and Western blotting but not frozen sections. This monoclonal antibody may also be used in ELISA techniques.
| frozen sections |
paraffin sections |
flow cytometry |
ELISA |
blocking |
Western Blotting |
| - |
+ |
+ |
- |
ND |
ND |
Fig. Immunohistochemical staining for CD138 (plasma cells) in lymph node using clone B-B4.
References:
1. Zhang et al. 1994. Blood, 83, 3654-3663,
2. Borset et al. 1993. Br.J.Hematol, 85, 446-451
3. Pellat et al..1994. Blood, 84, 2597-2603
4. Van Camp, B. et al. 1990. Blood, 76, 3777,
5. Harada, H. et al.. 1993. Blood, 81, 2658
6. Clement, C. et al.. Leucocyte Typing V, Boston, Eds. S.F. Schlossman et al. Oxford Univ. Press, p714-715, (1995).
For research only. Not for use in humans. For in vitro use only.
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