Application:
Monoclonal antibodies to MPO, clone 266.6K2 can be applied in flow cytometry, or in immuno-histochemistry using cytospots or frozen tissue sections.
Monoclonal antibodies to MPO (clone 266.6K2) are used for the diagnosis of AML (acute myeloid leukemia) (1-4). Non-myeloid leukemia (e.g. acute lymphoblastic leukemia) is usually non-reactive with anti-MPO antibodies. The combination of antibodies to MPO with IQ Starfiqs (for permeablization and fixation of peripheral blood leucocytes) enables the rapid determination of intracellular MPO by flow cytometry.
In a study comparing anti-MPO with other myeloid-reactive monoclonal antibodies such as CD13, CD14, CD15 and CD33, a higher diagnostic sensitivity and specificity was found for AML with anti-MPO. In most patients, more MPO-positive cells were detected by anti-MPO than by cytochemical staining with Sudan black and standard peroxidase methods (4).

Specifications:
Clone 266.6K2 produces mouse IgG1 monoclonal antibodies reactive with the intracellular enzyme MPO in peripheral blood granulocytes.

Performance Characteristics:
Staining with clone B-C3 (CD19) monoclonal antibody and analysis by flow cytometry is illustrated using a normal blood sample. Direct staining was performed using 10 ml of the PE-conjugated monoclonal antibody preparation and 100 ml of blood sample.

References:
1. Erber, W.M., et al, 1989. Lancet. 1. 761-5.
2. Davey,F.R., 1987. Am J. Hematol. 26. 157-66.
3. Majdic,O., et al, 1984. In.J.Cancer. 33. 617-623
4. Van der Schoot, C.E., 1990. Br.J Haematol. 74. 173-178
5. Griffin J.D., et al 1983. Blood. 62. 557-563.
6. Sabbath,K.D., J. Clin. Invest. 75. 746-753

For research only. Not for use in humans. For in vitro use only.