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Product Information CD95
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Introduction:
CD95, otherwise known as Fas ligand (FasL) is a type II transmembrane protein, which binds to Fas and induces a death program in cells expressing Fas (1-4). It is a 40 - 45 kDa protein expressed by activated lymphocytes, monocytes, neutrophils, fibroblasts and cell lines. FasL-mediated cytotoxicity is found in activated CD4+ T-helper type I cells, CD8+ cytotoxic T lymphocytes and hybridoma T cells (3-5). The activation of FasL gene in hybridoma T cells is highly sensitive to dexamethasone and cyclosporin A (6,7). The activation pathway for the expression of FasL cytotoxicity is initiated upon cross-linking the T cell receptor (TCR). This is followed by the degradation of IxBb, which leads to the induction of the immediate-early genes, the activation of FasL gene and finally the expression of FasL- mediated cytotoxicity (8). As a critical transcription factor for cell activation NF-xb can regulate FasL gene expression either by directly binding to FasL promoter or indirectly through immediate-early genes whose expression depends on NF-xb. NF-xb translocation is proteosome-dependent.
Application:
Abnormality in FasL gene has been linked to lymphoproliferative autoimmune disease (9).). FasL-mediated cytotoxicity has been shown to regulate T and B cell responses (10,11.) Forced expression of FasL in grafts may be responsible for transplantation tolerance (12) and drug-induced FasL expression in tumor cells may induce tumor cell apoptosis (13). While on the other hand, constitutive expression of FasL in tumor cells may help them evade immune surveillance by killing the infiltrating lymphocytes
Specifications:
Clone B-G30 is a mouse IgG1 monoclonal antibody which triggers apoptotic cell death (14)). It is applicable in flow cytometry and in vitro studies. It binds to up to 70% of peripheral monocytes and lymphocytes and to some human B and T cell lines.
Clone B-G27 is a IgG2a monoclonal antibody which triggers apoptotic cell death. It is suitable for use in flow cytometry and in vitro studies, immunohistochemistry and frozen sections (14). It binds to peripheral blood monocytes, some human B and T cell lines and several human myeloid cell lines.
Clone B-D29 is a mouse IgG1 monoclonal antibody which inhibits Fas ligand induced apoptosis. It is applicable in flow cytometry and in vitro studies (14). Clone B-D29 recognizes peripheral lymphocytes, and B and T cell lines.
Clone B-G34 is a mouse IgG1 monoclonal antibody which does not trigger apoptotic cell death. It may be used in flow cytometry and in vitro studies (14). Clone B-G34 recognizes epitopes on human lymphocytes and B and T cell lines.
Performance Characteristics:
An example of anti-CD95 staining is shown below. Activated lymphocytes (incubation with anti-CD3) were stained with clone B-G27 monoclonal antibodies (anti-CD95) and FITC conjugated secondary antibody and analyzed by flow cytometry
Clone B-G27, CD 95
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Subclass:
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IgG1
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Source:
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mouse
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Cellular expression:
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peripheral blood lymphocytes
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Positive cells:
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CD95 99.1%
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Mean fluorescence units:
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CD95 114
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References:
1. Hanabuchi,S., et al 1994. Proc.Natl. Acad. Sci. USA 91. 4930
2. Ju,S,T., et al 1994. Proc.Natl.Acad.Sci. USA 91.4185
3. Kagi,D., et al. 1994. Science. 265. 528
4. Lowin,B., et al. 1994. Nature. 370. 650
5. Rouvier, E., et al. 1993. J.Exp.Med. 177. 195
6. Dhein,J., et al. 1995. Nature. 373. 438
7. Ju, S.-T., et al 1995. Nature.373 444
8. Matsui,K., et al, 1997. Eur.J.Immunol. 2269-2278
9. Takahashi et al. 1994 Cell, 76, 969
10. Ozedemiri,M., et al 1996. Eur.J.Immunol. 26. 415;
11. Rothstein,T.L., et al 1995., Nature. 374. 163
12. Lau et al. 1996. Science 273. 109
13. Friesen,C., et al., 1996. Nature Med., 2. 574)
14. Vermot-Desroches, C., et al. 1996. CR 22.1 1/954. Kobe Leucocyte Typing Workshop VI.
For research only. Not for use in humans. For in vitro use only.
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