Application:
Monoclonal antibodies CD71, clone DF1513 can be applied in flow cytometry, in immuno-histochemistry on paraffin sections, cytospots or frozen tissue sections. It is also suitable for use in Western blotting. CD71 antibodies are used as an indicator of proliferation in the typing of lymphomas and leukemias (3)
CD71 plays a critical role in cell proliferation by controlling the supply of iron, which is essential for many metabolic pathways, through the binding and endocytosis of transferrin, the major iron-carrying protein (1). The expression of CD71 is regulated at the post-transcriptional level through the control of mRNA stability and is closely linked to intracellular iron levels (4).
Nitric oxide can affect CD71 expression independently of intracellular iron concentration by activating proteins which then stabilize CD71 mRNA (4).

Specifications:
CD71, clone DF1513, produces mouse IgG1 immunoglobulins directed against the transferrin receptor. The transferrin receptor is a type II membrane glycoprotein existing as a homodimer of 180-190kDa with the interchain disulphide bond. Clone DF1513 was clustered at the Leucocyte Typing Workshop IV (5).

Performance Characteristics:
CD71, clone DF1513 monoclonal antibodies were analyzed by flow cytometry using activated peripheral blood lymphocytes from a healthy donor. The cytogram shows direct staining with 10 ml CD71 FITC and 100 ml whole blood.

Clone DF1513, CD71

Subclass:	IgG1
Source:	mouse
Cellular expression:	activated leukocytes
Positive cells:	CD71 FITC 99.0 %
Mean fluorescence units:	CD71 FITC 91.9

References:
1. Testa,U., wt al 1993. Crit.Rev. Oncog. 4.241-276
2. Barclay,A.N., et al. 1997. Leucocyte Antigen FactsBook. Academic Press. London
3. Oudemans, et al. 1986 Cancer 58. 1252.
4. Hentze,M.W. and Kuhn,L.C. 1996. Proc.Natl Acad. Sci. USA 93. 8175-8182
5. Knapp,W., et al eds. 1989 Leucocyte Typing Workshop IV. Oxford University Press.

For research only. Not for use in humans. For in vitro use only.